Programa de Pós-Graduação em Ciências Biológicas - Fisiologia, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro-UFRJ, Rio de Janeiro, RJ, 21941-590, Brazil.
Programa de Pós-Graduação em Biologia Celular e do Desenvolvimento, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina-UFSC, João Pio Duarte Silva, 241, Florianópolis, SC, 88037-000, Brazil.
Cell Tissue Res. 2021 Apr;384(1):73-85. doi: 10.1007/s00441-021-03413-y. Epub 2021 Feb 18.
Neurogenesis occurs in adults of most organisms, both vertebrates and invertebrates. In semiterrestrial crabs of the infraorder Brachyura, the deutocerebrum, where neurogenesis occurs, processes the olfactory sensory information from the antennae. The deutocerebrum is composed of a pair of olfactory lobes associated with cell clusters 9 and 10 (Cl 9 and Cl 10), containing proliferating cells. Because the location of the neurogenic niche in brachyuran semiterrestrial crabs has not been defined, here we describe a neurogenic niche in the central olfactory system of the crab Ucides cordatus and report two types of glial cells in the deutocerebrum, based on different markers. Serotonin (5-hydroxytryptamine) labeling was used to reveal neuroanatomical aspects of the central olfactory system and the neurogenic niche. The results showed a zone of proliferating neural cells within Cl 10, which also contains III beta-tubulin (Tuj1)+ immature neurons, associated with a structure that has characteristics of the neurogenic niche. For the first time, using two glial markers, glial fibrillary acidic protein (GFAP) and glutamine synthetase (GS), we identified two types of astrocyte-like cells in different regions of the deutocerebrum. This study adds to the understanding of neurogenesis in a brachyuran semiterrestrial crustacean and encourages comparative studies between crustaceans and vertebrates, including mammals, based on shared aspects of both mechanisms of neurogenesis and regenerative potentials.
神经发生发生在大多数生物的成体中，包括脊椎动物和无脊椎动物。在短尾类十足目螃蟹的半陆生种类中，发生神经发生的后脑由一对与细胞簇 9 和 10（Cl 9 和 Cl 10）相关的嗅球组成，其中包含增殖细胞。由于短尾类半陆生螃蟹的神经发生龛的位置尚未确定，因此在这里我们描述了 Ucides cordatus 螃蟹中央嗅觉系统中的一个神经发生龛，并根据不同的标记物报告了后脑中的两种神经胶质细胞。血清素（5-羟色胺）标记用于揭示中央嗅觉系统和神经发生龛的神经解剖学方面。结果显示，Cl 10 内存在一个增殖神经细胞区，其中还包含 III beta-微管蛋白（Tuj1）+未成熟神经元，与具有神经发生龛特征的结构相关。首次使用两种神经胶质标记物胶质纤维酸性蛋白（GFAP）和谷氨酰胺合成酶（GS），我们在后脑的不同区域鉴定出两种类型的星形胶质细胞样细胞。这项研究增加了对半陆生十足目甲壳动物神经发生的理解，并鼓励基于神经发生机制和再生潜力的共同方面，在甲壳动物和脊椎动物（包括哺乳动物）之间进行比较研究。
