Ming C H, Fan Y S
Department of Physiology, Faculty of Medicine, University of Malaya, Kuala Lumpur.
J Immunol Methods. 1988 May 9;109(2):253-5. doi: 10.1016/0022-1759(88)90250-5.
Addition of the non-ionic detergent Tween 20 to the serum diluent enhances anti-cardiolipin binding reactivity in an ELISA system. Maximal enhancement was obtained using a concentration of 0.05% Tween 20 in the diluent. Non-specific interactions were also considerably reduced.
在血清稀释剂中添加非离子去污剂吐温20可增强酶联免疫吸附测定(ELISA)系统中抗心磷脂的结合反应性。在稀释剂中使用0.05%的吐温20浓度可获得最大增强效果。非特异性相互作用也显著减少。