Evaluation of an 2-Substituted (1-3)-β-D-Glucan, Produced by 2.6, in Models of Crohn's Disease.

1,3-β-glucans are extracellular polysaccharides synthesized by microorganisms and plants, with therapeutic potential. Among them, the 2-substituted-(1-3)-β-D-glucan, synthesized by some lactic acid bacteria (LAB), has a prebiotic effect on probiotic strains, an immunomodulatory effect on monocyte-derived macrophages, and potentiates the ability of the producer strain to adhere to Caco-2 cells differentiated to enterocytes. In this work, the 2-substituted-(1-3)-β-D-glucan polymers produced by GTF glycoyltransferase in the natural host 2.6 and in the recombinant strain NZ9000[pNGTF] were tested. Their immunomodulatory activity was investigated in an model using human biopsies from patients affected by Crohn's disease (CD). Both polymers had an anti-inflammatory effect including, a reduction of Interleukine 8 both at the level of its gene expression and its secreted levels. The overall data indicate that the 2-substituted-(1-3)-β-D-glucan have a potential role in ameliorating inflammation the gut immune system cell modulation.