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2-位取代的(1→3)-β-D-葡聚糖在乳酸乳球菌中的异源表达。

Heterologous expression of a position 2-substituted (1-->3)-beta-D-glucan in Lactococcus lactis.

作者信息

Werning María Laura, Corrales María Angeles, Prieto Alicia, de Palencia Pilar Fernández, Navas Jesús, López Paloma

机构信息

Departamento de Ciencia de Proteínas, Centro de Investigaciones Biológicas, Ramiro de Maeztu 9, 28040 Madrid, Spain.

出版信息

Appl Environ Microbiol. 2008 Aug;74(16):5259-62. doi: 10.1128/AEM.00463-08. Epub 2008 Jun 20.

DOI:10.1128/AEM.00463-08
PMID:18567684
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2519285/
Abstract

Exopolysaccharides play an important role in the rheology and texture of fermented foods, and among these beta-glucans have immunomodulating properties. We show that the overproduction of the Pediococcus parvulus GTF glycosyltransferase in an uncapsulated Lactococcus lactis strain results in synthesis and secretion (300 mg liter(-1)) of a position 2-substituted (1-->3)-beta-D-glucan that has potential use as a food additive.

摘要

胞外多糖在发酵食品的流变学和质地方面发挥着重要作用，其中β-葡聚糖具有免疫调节特性。我们发现，在无荚膜乳酸乳球菌菌株中过量表达小嗜热栖热菌GTF糖基转移酶会导致合成并分泌（300毫克/升）2位取代的（1→3）-β-D-葡聚糖，该葡聚糖有潜力用作食品添加剂。

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