Liu Chenyang, Zhang Qianqian, Zhu Hong, Cai Chunmei, Li Shuai
Key Laboratory of Plant Biotechnology in Universities of Shandong Province, College of Life Sciences, Qingdao Agricultural University, Qingdao, China.
College of Agronomy, Qingdao Agricultural University, Qingdao, China.
Front Plant Sci. 2021 Feb 4;12:608603. doi: 10.3389/fpls.2021.608603. eCollection 2021.
() genes play important roles in the regulation of plant growth and development, and they have been analyzed in many plant species. However, few studies have examined genes in mungbean (). In this study, we identified and characterized 31 mungbean genes whose proteins contained B-Box domains. Fourteen were designated as genes and were distributed on 7 of the 11 mungbean chromosomes. Based on their phylogenetic relationships, were clustered into three groups (I, II, and III), which contained 4, 6, and 4 members, respectively. The gene structures and conserved motifs of the genes were analyzed, and two duplicated gene pairs, and , were identified. A total of 82 -acting elements were found in the promoter regions, and the numbers and types of -acting elements in each promoter region differed. As a result, the expression patterns of varied in different tissues and throughout the day under long-day and short-day conditions. Among these genes, showed a close phylogenetic relationship with and displayed daily oscillations in expression under short-day conditions but not long-day conditions. In addition, overexpression of accelerated flowering in under short-day conditions by affecting the expression of the flowering time genes and . Our study lays the foundation for further investigation of gene functions.
（）基因在植物生长发育调控中发挥重要作用，并且已在许多植物物种中进行了分析。然而，很少有研究对绿豆（）中的基因进行研究。在本研究中，我们鉴定并表征了31个绿豆基因，其蛋白质含有B-Box结构域。其中14个被指定为基因，分布在11条绿豆染色体中的7条上。基于它们的系统发育关系，被聚类为三组（I、II和III），分别包含4、6和4个成员。分析了基因的基因结构和保守基序，并鉴定出两对重复基因，和。在基因的启动子区域共发现82个顺式作用元件，每个基因启动子区域中顺式作用元件的数量和类型各不相同。结果，基因的表达模式在不同组织以及长日和短日条件下全天都有所不同。在这些基因中，与显示出密切的系统发育关系，并且在短日条件下而非长日条件下表现出每日表达振荡。此外，在短日条件下，通过影响开花时间基因和的表达，的过表达加速了的开花。我们的研究为进一步研究基因功能奠定了基础。
