Department of Materials Science & Engineering, Stanford University.
Department of Chemical Engineering, Stanford University.
J Vis Exp. 2021 Feb 7(168). doi: 10.3791/62234.
These methods describe how to formulate injectable, supramolecular polymer-nanoparticle (PNP) hydrogels for use as biomaterials. PNP hydrogels are composed of two components: hydrophobically modified cellulose as the network polymer and self-assembled core-shell nanoparticles that act as non-covalent cross linkers through dynamic, multivalent interactions. These methods describe both the formation of these self-assembled nanoparticles through nanoprecipitation as well as the formulation and mixing of the two components to form hydrogels with tunable mechanical properties. The use of dynamic light scattering (DLS) and rheology to characterize the quality of the synthesized materials is also detailed. Finally, the utility of these hydrogels for drug delivery, biopharmaceutical stabilization, and cell encapsulation and delivery is demonstrated through in vitro experiments to characterize drug release, thermal stability, and cell settling and viability. Due to its biocompatibility, injectability, and mild gel formation conditions, this hydrogel system is a readily tunable platform suitable for a range of biomedical applications.
这些方法描述了如何制备可注射的超分子聚合物-纳米颗粒（PNP）水凝胶作为生物材料。PNP 水凝胶由两部分组成：疏水改性纤维素作为网络聚合物和自组装的核壳纳米粒子，通过动态、多价相互作用作为非共价交联剂。这些方法既描述了通过纳米沉淀形成这些自组装纳米粒子的方法，也描述了将两种成分混合形成具有可调节机械性能的水凝胶的方法。还详细介绍了使用动态光散射（DLS）和流变学来表征合成材料质量的方法。最后，通过体外实验来表征药物释放、热稳定性以及细胞沉降和活力，展示了这些水凝胶在药物输送、生物制药稳定和细胞封装与输送方面的应用。由于其生物相容性、可注射性和温和的凝胶形成条件，这种水凝胶系统是一个易于调节的平台，适用于多种生物医学应用。
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