Department of Medical Laboratory Science, Menelik Medical and Health Science College, Kotebe Metropolitan University, P.O. Box 3268, Addis Ababa, Ethiopia.
Department of Nutrition Science, MMHSC, KMU, Addis Ababa, Ethiopia.
BMC Complement Med Ther. 2021 Feb 23;21(1):74. doi: 10.1186/s12906-021-03249-7.
Medicinal plants have wide medicament application used to prevent and management of many ailments. These plants are used for primary health care in pastoralist communities who are deprived of modern medical care. They possess extensive therapeutics bioactive coupled with varied chemical structures. However, scientific validation of efficacy and safety of plants used to treat the urinary tract infections haven't been fully exploited. The aim of this study was to evaluate antimicrobial activity and screening phytochemicals of medicinal plants used to treat urinary tract infections.
In-vitro experimental study was carried out to evaluate the antimicrobial effect and screening phytochemical of Rumex abyssinicus, Cucumis pustulatus, Discopodium penninervium, Lippia adoensis, Euphorbia depauperata, and Cirsium englerianum. Against drug resistance microbes. 80% methanol was used for extraction of the plant parts. The susceptibility tests were investigated using disc diffusion and broth micro-dilution methods.
The majority of tested extracts showed antimicrobial activity on two or more drug-resistant bacteria with MIC value (1.0-128.0 μg/ml) and 9-27 mm inhibition zone in diameter. Extracts obtained from C.englerianum and E. depauperate showed more potent antibacterial activity on MRSA and Enterococcus faecalis with IZ 25 and 27 mm respectively. E. coli and K. pneumoniae were inhibited by those extracts with IZ ranging 9-25 mm and 11-27 mm respectively. E.faecalis and K. pneumoniae were more susceptible bacteria to the respective extracts. R. abyssinicus showed promising antifungal effect with had 21 mm IZ and MIC range 16-32 μg/ml on C.albicans. Alkaloids, flavonoids, phenolic and terpenoid were common phytochemical characterized in majority of screened plants.
Tested extracts exhibited significant antibacterial and antifungal activity. Hence, further structural elucidation of bioactive that inhibited the growth of microbes aforementioned plants may be used as precursors for the synthesis of new antibiotics in the future.
药用植物具有广泛的药用应用，可用于预防和治疗许多疾病。这些植物被用于缺乏现代医疗保健的牧民社区的初级保健。它们具有广泛的治疗生物活性，加上各种化学结构。然而，用于治疗尿路感染的植物的功效和安全性的科学验证尚未得到充分利用。本研究的目的是评估用于治疗尿路感染的药用植物的抗菌活性和筛选植物化学物质。
进行了体外实验研究，以评估 Rumex abyssinicus、Cucumis pustulatus、Discopodium penninervium、Lippia adoensis、Euphorbia depauperata 和 Cirsium englerianum 等药用植物的抗菌效果和筛选植物化学物质。针对耐药微生物。80%甲醇用于提取植物部分。使用圆盘扩散和肉汤微量稀释法进行了药敏试验。
大多数测试提取物对两种或更多种耐药细菌具有抗菌活性，MIC 值（1.0-128.0μg/ml）和直径为 9-27mm 的抑菌圈。从 C.englerianum 和 E. depauperate 获得的提取物对 MRSA 和粪肠球菌表现出更强的抗菌活性，抑菌圈分别为 25 和 27mm。那些提取物对大肠杆菌和肺炎克雷伯菌的抑制作用分别为 9-25mm 和 11-27mm。E.faecalis 和 K.pneumoniae 对各自的提取物更敏感。R.abyssinicus 对白色念珠菌表现出有希望的抗真菌作用，抑菌圈为 21mm，MIC 范围为 16-32μg/ml。生物碱、黄酮类、酚类和萜类是大多数筛选植物中常见的植物化学物质。
测试提取物表现出显著的抗菌和抗真菌活性。因此，对抑制上述微生物生长的生物活性物质的进一步结构阐明可能被用作未来合成新抗生素的前体。
