一种串联巨磁电阻检测法，可一次性定量检测人血液中临床相关浓度的 N 末端 pro-B 型利钠肽。

A tandem giant magnetoresistance assay for one-shot quantification of clinically relevant concentrations of N-terminal pro-B-type natriuretic peptide in human blood.

机构信息

Institute of Basic Medicine, The First Affiliated Hospital of Shandong First Medical University, Jinan, 250014, China.

Institute of Basic Medicine, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, 250062, China.

出版信息

Anal Bioanal Chem. 2021 May;413(11):2943-2949. doi: 10.1007/s00216-021-03227-5. Epub 2021 Feb 23.

DOI:10.1007/s00216-021-03227-5

PMID:33624128

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8043887/

Abstract

We report a microfluidic sandwich immunoassay constructed around a dual-giant magnetoresistance (GMR) sensor array to quantify the heart failure biomarker NT-proBNP in human plasma at the clinically relevant concentration levels between 15 pg/mL and 40 ng/mL. The broad dynamic range was achieved by differential coating of two identical GMR sensors operated in tandem, and combining two standard curves. The detection limit was determined as 5 pg/mL. The assay, involving 53 plasma samples from patients with different cardiovascular diseases, was validated against the Roche Cobas e411 analyzer. The salient features of this system are its wide concentration range, low detection limit, small sample volume requirement (50 μL), and the need for a short measurement time of 15 min, making it a prospective candidate for practical use in point of care analysis.

摘要

我们报告了一种基于双巨磁电阻（GMR）传感器阵列的微流控三明治免疫分析方法，用于在临床相关浓度范围内（15 pg/mL 至 40 ng/mL）定量检测人心肌肽前体 NT-proBNP 这一心衰生物标志物。通过对两个相同的 GMR 传感器进行差分涂层并结合两条标准曲线，实现了宽动态范围。检测限确定为 5 pg/mL。该检测方法涉及 53 名患有不同心血管疾病的患者的血浆样本，并用罗氏 Cobas e411 分析仪进行了验证。该系统的突出特点是其宽浓度范围、低检测限、小样本量需求（50 μL）以及需要 15 分钟的短测量时间，使其成为即时护理分析中实际应用的有前途的候选者。