Ewert A, Emerson G A
Am J Trop Med Hyg. 1979 May;28(3):496-9. doi: 10.4269/ajtmh.1979.28.496.
Sixty-three experimental and 58 control cats were infected with Brugia malayi so that the developing and adult worms localized in the regional lymphatics of the hind legs. At 20 days after infection when Brugia were in the 4th larval stage, and at 8 weeks when worms were young adults, cats were divided into groups to test the efficacy of diethylcarbamazine citrate (DEC) at various dosage levels. At 100 mg total DEC/kg no 4th-stage larvae were seen in 5 cats compared with a mean of 20.4 living larvae in each of 5 controls. At this level of DEC, 2 of 5 cats had 1 and 2 adult worms while 4 of 4 controls had a mean of 23.2 living adult worms. At 50 and 25 mg/kg there was a substantial reduction of both 4th stage and adult worms when compared to controls. At 10 mg/kg, 4 of 6 cats had 4th-stage larvae but at a lower level (mean = 7.0) than in 6 controls (mean = 23.2). No reduction of either 4th-stage larvae or adult worms was seen at 1 mg/kg. This study establishes the efficacy of DEC against 4th-stage and adult Brugia malayi in cats, although considerably higher levels of the drug were required than the level previously determined to kill 3rd-stage larvae. It appears that the cat-B. malayi model will be an effective method to compare the efficacy of drugs against adult lymphatic-dwelling filariae.
63只实验猫和58只对照猫感染了马来布鲁线虫，使发育中的和成虫定位于后腿的局部淋巴管。在感染后20天，即马来布鲁线虫处于第4幼虫期时，以及在感染后8周，即蠕虫为年轻成虫时，将猫分成几组，以测试不同剂量水平的枸橼酸乙胺嗪（DEC）的疗效。在总剂量为100mg DEC/kg时，5只猫中未发现第4期幼虫，而5只对照猫平均每只中有20.4条活幼虫。在这个DEC水平下，5只猫中有2只分别有1条和2条成虫，而4只对照猫平均有23.2条活成虫。与对照组相比，在50mg/kg和25mg/kg时，第4期幼虫和成虫数量均大幅减少。在10mg/kg时，6只猫中有4只存在第4期幼虫，但数量（平均=7.0）低于6只对照猫（平均=23.2）。在1mg/kg时，未观察到第4期幼虫或成虫数量减少。这项研究证实了DEC对猫体内第4期和成虫期的马来布鲁线虫有效，尽管所需药物水平比先前确定的杀死第3期幼虫的水平要高得多。看来猫-马来布鲁线虫模型将是比较药物对成年淋巴寄生丝虫疗效的有效方法。
