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新型N-三甲基壳聚糖纳米球作为破伤风疫苗稳定剂和防腐剂的生物学特性及应用

Biological properties the novel application of N-trimethyl chitosan nanospheres as a stabilizer and preservative in tetanus vaccine.

作者信息

Ghalavand Majdedin, Saadati Mojtaba, Salimian Jafar, Abbasi Ebrahim, Hosseinzadeh Ghader, Gouvarchin Ghaleh Hadi Esmaeili, Ahmadi Ali

机构信息

Student Research Committee, Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Applied Virology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran.

出版信息

Clin Exp Vaccine Res. 2021 Jan;10(1):24-34. doi: 10.7774/cevr.2021.10.1.24. Epub 2021 Jan 31.

DOI:10.7774/cevr.2021.10.1.24
PMID:33628751
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7892940/
Abstract

PURPOSE

Chitosan is a natural polymer that has excellent properties include biocompatibility, biodegradability, no cytotoxicity, high charge density, low cost, mucoadhesive, permeation enhancing (ability to cross tight junction), and immunomodulating ability that makes the spectrum of its applicability much broader. This study was conducted to investigate the stabilizing, preservative and immunogenicity properties of N-trimethyl chitosan nanospheres (N-TMCNS).

MATERIALS AND METHODS

The tetanus toxoid (TT) was encapsulated into N-TMCNS and then characterized by scanning electron microscope, atomic force microscope, and dynamic light scattering. For stabilizer assay of N-TMCNS after storage of TT-N-TMCNS at different temperatures for 3 weeks, they were used for immunization of mice and different temperatures groups' anti-TT-N-TMCNS production compared with other groups. Finally, the immunized mice were challenged with tetanus toxin. The preservation activity of TT-N-TMCNS against was compared with thimerosal formulated TT.

RESULTS

Our results revealed that heat-treated TT-N-TMCNS could induce higher titer of neutralizing immunoglobulin G in compared to TT vaccine and was able to protect the mice better than TT vaccine in challenge test. Furthermore, N-TMCNS as a preservative inhibited the growth of more effective than thimerosal.

CONCLUSION

Overall, the obtained results indicated that the N-TMCNS is one of the best stabilizer and preservative agent that can be used in the formulation of TT vaccine.

摘要

目的

壳聚糖是一种天然聚合物，具有优异的性能，包括生物相容性、生物降解性、无细胞毒性、高电荷密度、低成本、粘膜粘附性、渗透增强（穿越紧密连接的能力）以及免疫调节能力，这使得其适用范围更广。本研究旨在调查N-三甲基壳聚糖纳米球（N-TMCNS）的稳定、防腐和免疫原性特性。

材料与方法

将破伤风类毒素（TT）包封于N-TMCNS中，然后通过扫描电子显微镜、原子力显微镜和动态光散射进行表征。为了在不同温度下储存TT-N-TMCNS 3周后对N-TMCNS进行稳定剂测定，将它们用于小鼠免疫，并将不同温度组的抗TT-N-TMCNS产生与其他组进行比较。最后，用破伤风毒素攻击免疫的小鼠。将TT-N-TMCNS对[此处原文缺失相关内容]的保存活性与硫柳汞配制的TT进行比较。

结果

我们的结果显示，与TT疫苗相比，热处理的TT-N-TMCNS能诱导更高滴度的中和免疫球蛋白G，并且在攻毒试验中比TT疫苗能更好地保护小鼠。此外，N-TMCNS作为防腐剂比硫柳汞更有效地抑制[此处原文缺失相关内容]的生长。

结论

总体而言，所得结果表明N-TMCNS是可用于TT疫苗制剂的最佳稳定剂和防腐剂之一。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/7892940/59723c149c48/cevr-10-24-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/7892940/758a964c67c6/cevr-10-24-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/7892940/1f4cc6d9cdec/cevr-10-24-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/7892940/bd2aea87b34e/cevr-10-24-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/7892940/e33b8622b4c6/cevr-10-24-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/7892940/c0733f114f6d/cevr-10-24-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/7892940/b3a6c2fd7e24/cevr-10-24-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/7892940/59723c149c48/cevr-10-24-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/7892940/758a964c67c6/cevr-10-24-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/7892940/1f4cc6d9cdec/cevr-10-24-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/7892940/bd2aea87b34e/cevr-10-24-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/7892940/e33b8622b4c6/cevr-10-24-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/7892940/c0733f114f6d/cevr-10-24-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/7892940/b3a6c2fd7e24/cevr-10-24-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c18/7892940/59723c149c48/cevr-10-24-g007.jpg

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