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对胃肠道疾病患者进行贾第虫病的显微镜检查和免疫诊断筛查。

A microscopic and immunodiagnostic search for giardiasis in patients with gastrointestinal disorders.

作者信息

Allison M C, Green E L, Bhattacharya D N, Smith A, Pounder R E

机构信息

Academic Dept. of Medicine, Royal Free Hospital, London, U.K.

出版信息

Scand J Gastroenterol. 1988 Mar;23(2):209-12. doi: 10.3109/00365528809103969.

DOI:10.3109/00365528809103969
PMID:3363292
Abstract

Direct microscopy and an ELISA technique were used to determine the prevalence of Giardia lamblia and its antigen in stool samples from patients with Crohn's disease, ulcerative colitis, acute-onset diarrhoea, or dyspepsia. Cysts of Giardia lamblia were observed by microscopy of faeces from two of the patients with acute-onset diarrhoea and one with dyspepsia. Giardia antigen was detected in the faeces of five patients, including all three in whom cysts had been identified by microscopy. No evidence of giardiasis was found in any patient with Crohn's disease or ulcerative colitis. It is concluded that the ELISA can reliably distinguish giardiasis from a range of other gastrointestinal disorders.

摘要

采用直接显微镜检查和酶联免疫吸附测定(ELISA)技术,以确定来自克罗恩病、溃疡性结肠炎、急性腹泻或消化不良患者粪便样本中贾第鞭毛虫及其抗原的流行情况。通过显微镜检查,在两名急性腹泻患者和一名消化不良患者的粪便中观察到了贾第鞭毛虫囊肿。在五名患者的粪便中检测到了贾第鞭毛虫抗原,其中包括通过显微镜检查发现囊肿的所有三名患者。在任何克罗恩病或溃疡性结肠炎患者中均未发现贾第虫病的证据。得出的结论是,ELISA能够可靠地将贾第虫病与一系列其他胃肠道疾病区分开来。

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引用本文的文献

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Laboratory diagnosis for Giardia lamblia infection: A comparison of microscopy, coprodiagnosis and serology.蓝氏贾第鞭毛虫感染的实验室诊断:显微镜检查、粪便诊断与血清学的比较
Can J Infect Dis. 1997 Jan;8(1):33-8. doi: 10.1155/1997/270179.
2
Evaluation of rapid commercial enzyme immunoassay for detection of Giardia lamblia in formalin-preserved stool specimens.评估快速商业酶免疫测定法在福尔马林固定粪便标本中检测蓝氏贾第鞭毛虫的效果。
J Clin Microbiol. 1994 Jul;32(7):1807-8. doi: 10.1128/jcm.32.7.1807-1808.1994.
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Application of a capture enzyme immunoassay in an outbreak of waterborne giardiasis in the United Kingdom.
捕获酶免疫测定法在英国一次水源性贾第虫病暴发中的应用。
Eur J Clin Microbiol Infect Dis. 1990 Jun;9(6):424-8. doi: 10.1007/BF01979475.