Scheffler E H, Van Etta L L
Department of Microbiology, Duluth Clinic, Minnesota 55805.
J Clin Microbiol. 1994 Jul;32(7):1807-8. doi: 10.1128/jcm.32.7.1807-1808.1994.
Two hundred twenty-three formalin-preserved stool specimens were evaluated by using ProSpecT Giardia Rapid Assay (membrane bound) (Alexon, Inc., Sunnyvale, Calif.). Enzyme immunoassay (EIA) results were compared with those by conventional microscopic examination. Two hundred four specimens were negative by both methods, and 13 (6.3%) were positive. Five specimens were negative by initial microscopic exam and positive by EIA; three of these specimens were found to be positive upon extensive microscopic reexamination. The remaining two specimens were from patients who previously tested positive and who had recurrent symptoms of or responded to therapy for giardia. Therefore, we consider both cases to be true positives. One specimen exhibited a single cyst by microscopic exam and was negative by EIA Resolved results yielded a relative sensitivity of 95%, a relative specificity of 100%, a positive predictive value of 99.6%, and a negative predictive value of 100%, compared with a sensitivity of 74% for conventional microscopy.
使用ProSpecT贾第鞭毛虫快速检测法（膜结合法）（Alexon公司，加利福尼亚州森尼韦尔市）对223份福尔马林固定的粪便标本进行了评估。将酶免疫分析（EIA）结果与传统显微镜检查结果进行了比较。两种方法均显示204份标本为阴性，13份（6.3%）为阳性。5份标本初次显微镜检查为阴性，但EIA为阳性；其中3份标本经广泛显微镜复查后被发现为阳性。其余2份标本来自先前检测呈阳性且有贾第鞭毛虫复发症状或对治疗有反应的患者。因此，我们认为这两例均为真阳性。1份标本显微镜检查发现单个包囊，但EIA为阴性。与传统显微镜检查74%的灵敏度相比，最终结果显示相对灵敏度为95%，相对特异性为100%，阳性预测值为99.6%，阴性预测值为100%。
