Department of Biomedical Sciences, Faculty of Medicine & Health Sciences, An-Najah National University, Nablus, Palestine.
Department of Pharmacy, Faculty of Medicine & Health Sciences, An-Najah National University, Nablus, Palestine.
Int J Nanomedicine. 2021 Feb 15;16:989-1000. doi: 10.2147/IJN.S289107. eCollection 2021.
Under certain conditions, the physiological repair of connective tissues might fail to restore the original structure and function. Optimized engineered connective tissues (ECTs) with biophysical properties adapted to the target tissue could be used as a substitution therapy. This study aimed to investigate the effect of ECT enforcement by a complex of multiwall carbon nanotubes with chitosan (C-MWCNT) to meet in vivo demands.
ECTs were constructed from human foreskin fibroblasts (HFF-1) in collagen type I and enriched with the three different percentages 0.025, 0.05 and 0.1% of C-MWCNT. Characterization of the physical properties was performed by biomechanical studies using unidirectional strain.
Supplementation with 0.025% C-MWCNT moderately increased the tissue stiffness, reflected by Young's modulus, compared to tissues without C-MWCNT. Supplementation of ECTs with 0.1% C-MWCNT reduced tissue contraction and increased the elasticity and the extensibility, reflected by the yield point and ultimate strain, respectively. Consequently, the ECTs with 0.1% C-MWCNT showed a higher resilience and toughness as control tissues. Fluorescence tissue imaging demonstrated the longitudinal alignment of all cells independent of the condition.
Supplementation with C-MWCNT can enhance the biophysical properties of ECTs, which could be advantageous for applications in connective tissue repair.
在某些情况下，结缔组织的生理修复可能无法恢复原始结构和功能。具有适应目标组织的生物物理特性的优化工程化结缔组织 (ECT) 可以用作替代治疗。本研究旨在研究通过壳聚糖多壁碳纳米管复合物 (C-MWCNT) 增强 ECT 以满足体内需求的效果。
ECT 由人包皮成纤维细胞 (HFF-1) 在 I 型胶原中构建，并分别用 0.025%、0.05%和 0.1%的三种不同百分比的 C-MWCNT 进行富集。通过单向应变的生物力学研究对物理性能进行了表征。
与不含 C-MWCNT 的组织相比，添加 0.025% C-MWCNT 可适度增加组织刚度，反映为杨氏模量。添加 0.1% C-MWCNT 的 ECT 减少了组织收缩，增加了弹性和伸展性，分别反映为屈服点和极限应变。因此，与对照组织相比，添加 0.1% C-MWCNT 的 ECT 具有更高的弹性和韧性。荧光组织成像显示，所有细胞均独立于条件呈纵向排列。
C-MWCNT 的添加可以增强 ECT 的生物物理特性，这对于结缔组织修复的应用可能是有利的。
