Hibrawi H, Garrison F D, Smith H J
J Immunol Methods. 1977;14(1):59-63. doi: 10.1016/s0022-1759(97)90022-3.
Twenty-two samples of commercial agarose were utilized as the gel matrix in a counter-immunoelectrophoresis (CIE) for detection of urinary myoglobin. When different samples were tested under identical conditions, significant differences in the detectable levels of myoglobin by CIE could be traced to the quality of the agarose used. The quality of agarose preparation could be improved by repurification using polyethylene glycol precipitation. The necessity of using purified agarose when assaying for urinary myoglobin by CIE is stressed. This is particularly important when the test is used in the early detection of myocardial infarcts.
二十二份市售琼脂糖样品被用作免疫对流电泳（CIE）检测尿肌红蛋白的凝胶基质。在相同条件下对不同样品进行检测时，CIE可检测到的肌红蛋白水平存在显著差异，这可归因于所用琼脂糖的质量。琼脂糖制剂的质量可通过聚乙二醇沉淀法再纯化得到改善。强调了在通过CIE检测尿肌红蛋白时使用纯化琼脂糖的必要性。当该检测用于心肌梗死的早期诊断时，这一点尤为重要。
