Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Collaborative Innovation Center for Mountain Ecology & Agro-Bioengineering (CICMEAB), College of Life Sciences, Institute of Agro-Bioengineering, Guizhou University, Guiyang, 550025, Guizhou, China.
Arch Microbiol. 2021 Jul;203(5):2279-2290. doi: 10.1007/s00203-021-02204-x. Epub 2021 Mar 1.
Plant growth-promoting rhizobacteria that produce 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase can promote plant growth and enhance abiotic stress tolerance. In this study, Burkholderia pyrrocinia strain P10, with an ACC deaminase activity of 33.01-µmol/h/mg protein, was isolated from the tea rhizosphere and identified based on morphological, biochemical, and molecular characteristics. In addition to its ACC deaminase activity at pH 5.0-9.0 and in response to 5% NaCl and 20% polyethylene glycol, strain P10 can also solubilize phosphorus compounds, produce indole-3-acetic acid, and secrete siderophores. Pot experiments revealed that strain P10 can significantly enhance peanut seedling growth under saline conditions (100- and 170-mmol/L NaCl). Specifically, it increased the fresh weight and root length of plants by 90.12% and 79.22%, respectively, compared with high-salt stress. These results provide new insights into the biological characteristics of Burkholderia pyrrocinia, which may be useful as a bio-fertilizer.
能够产生 1-氨基环丙烷-1-羧酸（ACC）脱氨酶的植物促生根际细菌可以促进植物生长并增强非生物胁迫耐受性。在这项研究中，从茶根际中分离出具有 ACC 脱氨酶活性为 33.01-µmol/h/mg 蛋白的伯克霍尔德氏菌（Burkholderia pyrrocinia）菌株 P10，并基于形态学、生物化学和分子特征进行了鉴定。除了在 pH 值为 5.0-9.0 以及对 5%NaCl 和 20%聚乙二醇的响应下具有 ACC 脱氨酶活性外，菌株 P10 还可以溶解磷化合物、产生吲哚-3-乙酸并分泌铁载体。盆栽实验表明，菌株 P10 可以在盐胁迫条件下（100-和 170-mmol/L NaCl）显著促进花生幼苗的生长。具体而言，与高盐胁迫相比，它分别使植物的鲜重和根长增加了 90.12%和 79.22%。这些结果为伯克霍尔德氏菌的生物学特性提供了新的见解，它可能作为生物肥料有用。
