Zhao Siyue, Li Caiwu, Zhu Tao, Jin Lei, Deng Wenwen, Zhao Ke, He Yongguo, Li Guo, Xiong Yaowu, Li Ti, Li Bei, Huang Yan, Zhang Hemin, Zou Likou
College of Resources, Sichuan Agricultural University, Chengdu, Sichuan, China.
Key Laboratory of SFGA on Conservation Biology of Rare Animals in the Giant Panda National Park, China Conservation and Research Center for Giant Panda, Dujiangyan, Sichuan, China.
Curr Microbiol. 2021 Apr;78(4):1358-1366. doi: 10.1007/s00284-021-02424-w. Epub 2021 Mar 1.
The giant panda (GP) is the most precious animal in China. Gastrointestinal tract disease, especially associated with dysbiosis of gut microbiota, is the leading cause of death in GPs. Here, we performed 16S rRNA high-throughput sequencing to investigate the gut microbiota of GPs having symptoms of anorexia. Results showed that gut microbiota of GP with anorexia had lower richness (Chao1 index) than the healthy GP. However, no significant differences in alpha diversity were observed. There is a significance in the microbial structure between anorexia and healthy GPs. The abundance of phylum Firmicutes (99.23% ± 7.1%), unidentified genus Clostridiales (24.75% ± 2.5%), was significantly higher in the subadult anorexia group (P < 0.01), and that of the unidentified genus Clostridiales (4.53% ± 1.2%) was also significantly higher in the adult anorexia group (P < 0.01). Weissella and Streptococcus were found to be decreased in both anorexia groups. The decreased abundance of Weissella (0.02% ± 0.0%, 0.08% ± 0.0%) and Streptococcus (73.89% ± 4.3%, 91.15% ± 7.6%) and increase in Clostridium may cause symptoms of anorexia in giant pandas. The correlation analysis indicated that there is a symbiotic relationship among Streptococcus, Leuconostoc, Weissella, and Bacillus which are classified as probiotics (r > 0.6, P < 0.05). Importantly, a negative correlation has been found between Streptococcus and unidentified_Clostridium in two groups (r > 0.6, P < 0.05). Our results suggested that Streptococcus might be used as probiotics to control the growth of Clostridium causing the anorexia.
大熊猫是中国最珍贵的动物。胃肠道疾病，尤其是与肠道微生物群失调相关的疾病，是大熊猫死亡的主要原因。在此，我们进行了16S rRNA高通量测序，以研究有厌食症状的大熊猫的肠道微生物群。结果显示，有厌食症状的大熊猫的肠道微生物群丰富度（Chao1指数）低于健康大熊猫。然而，未观察到α多样性的显著差异。厌食大熊猫和健康大熊猫之间的微生物结构存在显著差异。在亚成年厌食组中，厚壁菌门（99.23%±7.1%）、未鉴定的梭菌目属（24.75%±2.5%）的丰度显著更高（P<0.01），在成年厌食组中，未鉴定的梭菌目属（4.53%±1.2%）的丰度也显著更高（P<0.01）。在两个厌食组中均发现魏斯氏菌属和链球菌属减少。魏斯氏菌属（0.02%±0.0%，0.08%±0.0%）和链球菌属（73.89%±4.3%，91.15%±7.6%）丰度的降低以及梭菌属的增加可能导致大熊猫出现厌食症状。相关性分析表明，被归类为益生菌的链球菌属、明串珠菌属、魏斯氏菌属和芽孢杆菌属之间存在共生关系（r>0.6，P<0.05）。重要的是，在两组中均发现链球菌属与未鉴定的梭菌属之间存在负相关（r>0.6，P<0.05）。我们的结果表明，链球菌属可能用作益生菌来控制导致厌食的梭菌属的生长。
