112 Lab, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing 100083, China.
Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment for Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences, Dongcheng District, Beijing 100700, China.
Int J Biol Macromol. 2021 May 1;178:229-239. doi: 10.1016/j.ijbiomac.2021.02.126. Epub 2021 Feb 27.
The construction of protein abundance profiles helps to interpret the clinical applications of stem cells. Dental pulp stem cells (DPSCs) and gingival mesenchymal stem cells (GMSCs) can be isolated from teeth and used as a highly convenient clinical potential material. Here, we aimed to explore commonalities and differences of DPSCs and GMSCs at the protein level. TMT-based quantitative proteomics and two-dimensional gel electrophoresis technology were used in combination to describe the protein profile of DPSCs and GMSCs extracted from the same donor. A total of 2821 proteins were identified by LC-MS/MS, of which 248 differentially abundant proteins (DAPs) were highly expressed in GMSCs while 782 proteins were highly expressed in DPSCs. The biological functions and molecular pathways of DAPs were annotated with GO enrichment and KEGG analysis. The relationship between molecular abundance and cell characteristics including source, proliferation, angiogenesis and inflammation were connected by WGCNA. Special markers, including Calreticulin (CALR), Annexin A5 (ANXA5) and Rho GDP dissociation inhibitor alpha (GDIR1), were proposed to distinguish DPSCs from GMSCs. Our results provide a molecular basis for in-depth understanding of the protein composition and special functions of dental stem cells, and promote the potential clinical application.
蛋白质丰度谱的构建有助于解释干细胞的临床应用。牙髓干细胞（DPSCs）和牙龈间充质干细胞（GMSCs）可从牙齿中分离出来，作为一种极具临床应用潜力的材料。本研究旨在探索同一供体来源的 DPSCs 和 GMSCs 在蛋白质水平上的共性和差异。采用 TMT 定量蛋白质组学和二维凝胶电泳技术相结合的方法，描述了从 DPSCs 和 GMSCs 中提取的蛋白质图谱。通过 LC-MS/MS 鉴定出 2821 种蛋白质，其中 248 种差异丰度蛋白（DAPs）在 GMSCs 中高表达，而 782 种蛋白质在 DPSCs 中高表达。对 DAPs 的生物学功能和分子途径进行了 GO 富集和 KEGG 分析注释。通过 WGCNA 将分子丰度与细胞特性（包括来源、增殖、血管生成和炎症）之间的关系进行了关联。提出了特殊标志物，包括钙网蛋白（CALR）、膜联蛋白 A5（ANXA5）和 Rho GDP 解离抑制剂α（GDIR1），用于区分 DPSCs 和 GMSCs。本研究结果为深入了解牙齿干细胞的蛋白质组成和特殊功能提供了分子基础，并促进了其潜在的临床应用。
