在微生物系统中对植物细胞色素 P450 进行功能重建的合理设计策略。
Rational design strategies for functional reconstitution of plant cytochrome P450s in microbial systems.
机构信息
Department of Chemical and Environmental Engineering, University of California, Riverside, CA 92521, USA.
Disruptive & Sustainable Technologies for Agricultural Precision, Singapore-MIT Alliance for Research and Technology, Singapore; Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore.
出版信息
Curr Opin Plant Biol. 2021 Apr;60:102005. doi: 10.1016/j.pbi.2021.102005. Epub 2021 Feb 26.
Plant natural products (NPs) are of pharmaceutical and agricultural significance, yet the low abundance is largely impeding the broad investigation and utilization. Microbial bioproduction is a promising alternative sourcing to plant NPs. Cytochrome P450s (CYPs) play an essential role in plant secondary metabolism, and functional reconstitution of plant CYPs in the microbial system is one of the major challenges in establishing efficient microbial plant NP bioproduction. In this review, we briefly summarized the recent progress in rational engineering strategies for enhanced activity of plant CYPs in Escherichia coli and Saccharomyces cerevisiae, two commonly used microbial hosts. We believe that in-depth foundational investigations on the native microenvironment of plant CYPs are necessary to adapt the microbial systems for more efficient functional reconstitution of plant CYPs.
植物天然产物（NPs）具有药用和农业意义，但由于其丰度低，在很大程度上阻碍了广泛的研究和利用。微生物生物生产是一种有前途的植物 NPs 替代来源。细胞色素 P450s（CYPs）在植物次生代谢中起着重要作用，在微生物系统中对植物 CYP 的功能重建是建立有效的微生物植物 NP 生物生产的主要挑战之一。在这篇综述中，我们简要总结了近年来在提高大肠杆菌和酿酒酵母这两种常用微生物宿主中植物 CYP 活性的合理工程策略方面的最新进展。我们相信，对植物 CYP 天然微环境的深入基础研究对于使微生物系统更有效地进行植物 CYP 的功能重建是必要的。
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