School of Bioengineering, Dalian University of Technology, Dalian 116024, China.
College of Life Science, Dalian Minzu University, Dalian 116600, China; Key Laboratory of Biotechnology and Bioesources Utilization, Ministry of Education, Dalian 116600, China.
Food Res Int. 2021 Feb;140:110058. doi: 10.1016/j.foodres.2020.110058. Epub 2020 Dec 24.
The mechanism of response of plant to wounding stress is a complex process that physiologically modifies the wounded tissue for protection. In this study, untargeted metabolomics and physiological analyses were performed to validate the molecular mechanism of response to wounding stress of two intensities (0.04 and 1.85 m kg) in broccoli florets and shreds, respectively. The results showed that 97 and 1220 differentially expressed metabolites could be identified in broccoli subjected to the Florets vs. Control and Shreds vs. Control experiments, respectively. The Kyoto Encyclopedia Genes and Genomes pathway analyses revealed that these metabolites were mainly involved in aminoacyl-tRNA, amino acid, and secondary metabolite biosynthesis; purine metabolism; and plant signal molecule production. This study validated that wounding stress induced plant signal molecule production. Activation of jasmonic acid biosynthesis and HO production were more susceptible to wounding stress of higher intensities, whereas induction of salicylic acid biosynthesis and O production were more susceptible to wounding stress of lower intensities. Furthermore, wounding stress also activated glucosinolate and phenylpropanoid biosynthesis by regulating the levels of the precursors, including L-leucine, phenylalanine, tyrosine, valine, isoleucine, tryptophan, methionine, and phenylalanine. Wounding stress induced phenylpropanoid biosynthesis and the antioxidant system by upregulating the corresponding critical enzyme activity and gene expression, contributing greatly to the enhancement of phenolic compound levels, free radical scavenging ability, and resistance to wounding in broccoli.
植物对创伤应激的反应机制是一个复杂的过程，它在生理上修饰受伤组织以进行保护。在这项研究中，我们分别对花椰菜小花和碎块进行了非靶向代谢组学和生理分析，以验证两种强度（0.04 和 1.85 m kg）创伤应激反应的分子机制。结果表明，在小花与对照和碎块与对照的实验中，分别可以鉴定出 97 和 1220 个差异表达代谢物。京都基因与基因组百科全书通路分析表明，这些代谢物主要涉及氨酰-tRNA、氨基酸和次生代谢物生物合成；嘌呤代谢；和植物信号分子的产生。这项研究验证了创伤应激会诱导植物信号分子的产生。茉莉酸生物合成和 HO 生成的激活对高强度的创伤应激更敏感，而水杨酸生物合成和 O 生成的诱导对低强度的创伤应激更敏感。此外，创伤应激还通过调节 L-亮氨酸、苯丙氨酸、酪氨酸、缬氨酸、异亮氨酸、色氨酸、蛋氨酸和苯丙氨酸等前体的水平，激活了硫代葡萄糖苷和苯丙烷生物合成。创伤应激通过上调相应关键酶的活性和基因表达诱导苯丙烷生物合成和抗氧化系统，极大地促进了酚类化合物水平、自由基清除能力和花椰菜对创伤的抗性的提高。
