Bhagwan Mahavir College of Pharmacy, Surat, India.
Vital Care Pvt Ltd, Vadodara, India.
Folia Med (Plovdiv). 2021 Feb 28;63(1):67-73. doi: 10.3897/folmed.63.e49167.
Thrombocytopenia is a condition characterized by abnormally low levels of thrombocytes, also known as platelets, in the blood. Several medicinal plants possess curative and protective effect against thrombocytopenia associated with diseases or drugs.
In the present study, we have investigated the platelet augmentation activity of polyherbal formulation (VITA PLAT Capsule) in cyclophosphamide-induced thrombocytopenic rat model.
Twenty-four albino Wistar rats were divided into four groups. Thrombocytopenia was induced in the rats by administering cyclophosphamide (25 mg/kg, i.p.) for three days to all the groups except normal controls. The test groups were given orally a polyherbal formulation suspended in normal saline for 14 days. Blood was withdrawn from the retro-orbital plexus of the rats on days 1, 7, and 14 of study to determine platelet counts in all groups. Clotting time and bleeding time were determined on the last day of study. Data were collected and analyzed using GraphPad Prism 8.
The results showed that the polyherbal formulation treatment could significantly ameliorate platelet count in thrombocyto-penic rats in the initial as well as in the later phase. The total WBC count was also improved during later phase in test groups. However, there is no significant difference between clotting time and bleeding time in all groups.
Our study suggests a potential role of this formulation in the augmentation of platelet counts in various thrombocyto-penic disorders including a role in ameliorating the haemorrhagic complications of dengue fever.
血小板减少症是一种特征为血液中血小板（也称为血小板）水平异常降低的病症。有几种药用植物对与疾病或药物相关的血小板减少症具有治疗和保护作用。
在本研究中，我们研究了复方草药制剂（VITA PLAT 胶囊）在环磷酰胺诱导的血小板减少症大鼠模型中的血小板增加活性。
将 24 只白化 Wistar 大鼠分为四组。除正常对照组外，所有组的大鼠均通过腹腔注射环磷酰胺（25mg/kg）连续三天诱导血小板减少症。实验组连续 14 天口服混悬于生理盐水的复方草药制剂。在研究的第 1、7 和 14 天从大鼠眶后丛采血，以确定所有组的血小板计数。在研究的最后一天测定凝血时间和出血时间。使用 GraphPad Prism 8 收集和分析数据。
结果表明，复方制剂治疗可显著改善血小板减少症大鼠的血小板计数，无论是在初始阶段还是在后期阶段。在试验组中，白细胞总数在后期阶段也有所改善。然而，所有组的凝血时间和出血时间之间没有显著差异。
我们的研究表明，该制剂在各种血小板减少症中增加血小板计数方面具有潜在作用，包括在改善登革热出血并发症方面的作用。
