Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture and Rural Affairs, State Key Laboratory of Livestock and Poultry Breeding, Guangdong Provincial Key Laboratory of Animal Breeding and Nutrition, Maoming Branch of Guangdong Laboratory for Lingnan Modern Agriculture, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Guangdong Key Laboratory for Crop Germplasm Resources Preservation and Utilization, Agro-Biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Curr Microbiol. 2021 Apr;78(4):1648-1655. doi: 10.1007/s00284-021-02431-x. Epub 2021 Mar 2.
Phycosphere hosts the boundary of unique holobionts harboring dynamic algae-bacteria interactions. During our investigating the microbial consortia composition of phycosphere microbiota (PM) derived from diverse harmful algal blooms (HAB) dinoflagellates, a novel rod-shaped, motile and faint yellow-pigmented bacterium, designated as strain LZ-6, was isolated from HAB Alexandrium catenella LZT09 which produces high levels paralytic shellfish poisoning toxins. Phylogenetic analysis based on 16S rRNA gene and two housekeeping genes, rpoA and pheS sequences showed that the novel isolate shared the highest gene similarity with Marinobacter shengliensis CGMCC 1.12758 (99.6%) with the similarity values of 99.6%, 99.9% and 98.5%, respectively. Further phylogenomic calculations of average nucleotide identity (ANI), average amino acid identity (AAI) and digital DNA-DNA hybridization (dDDH) values between strains LZ-6 and the type strain of M. shengliensis were 95.9%, 96.4% and 68.5%, respectively. However, combined phenotypic and chemotaxonomic characterizations revealed that the new isolate was obviously different from the type strain of M. shengliensis. The obtained taxonomic evidences supported that strain LZ-6 represents a novel subspecies of M. shengliensis, for which the name is proposed, Marinobacter shengliensis subsp. alexandrii subsp. nov. with the type strain LZ-6 (= CCTCC AB 2018388T = KCTC 72197). This proposal automatically creates Marinobacter shengliensis subsp. shengliensis for which the type strain is SL013A34A2 (= LMG 27740 = CGMCC 1.12758).
藻际是独特的后生动物的边界，其中包含动态的藻类-细菌相互作用。在研究来自不同赤潮甲藻的藻际微生物群（PM）的微生物群落组成时，从产生高浓度麻痹性贝类毒素的赤潮亚历山大藻 LZT09 中分离到一种新型杆状、运动和淡黄色的细菌，命名为 LZ-6 菌株。基于 16S rRNA 基因和两个管家基因 rpoA 和 pheS 序列的系统发育分析表明，新分离株与海洋芽孢杆菌 CGMCC 1.12758（99.6%）具有最高的基因相似性，相似性值分别为 99.6%、99.9%和 98.5%。进一步对 LZ-6 菌株与海洋芽孢杆菌的型株进行全基因组计算平均核苷酸同一性（ANI）、平均氨基酸同一性（AAI）和数字 DNA-DNA 杂交（dDDH）值，分别为 95.9%、96.4%和 68.5%。然而，结合表型和化学分类特征表明，新分离株与海洋芽孢杆菌的型株明显不同。获得的分类学证据支持 LZ-6 菌株代表海洋芽孢杆菌的一个新亚种，命名为海洋芽孢杆菌亚历山大亚种 nov. subsp. alexandrii，其模式菌株为 LZ-6（= CCTCC AB 2018388T = KCTC 72197）。这一建议自动创建了海洋芽孢杆菌亚种 shengliensis，其模式菌株为 SL013A34A2（= LMG 27740 = CGMCC 1.12758）。
