Division of Environmental Science & Ecological Engineering, College of Life Science & Biotechnology, Korea University, Seoul 02841, Republic of Korea.
School of Earth and Environmental Science & Research Institute of Oceanography, Seoul National University, Seoul, Republic of Korea.
Int J Syst Evol Microbiol. 2020 Dec;70(12):6294-6300. doi: 10.1099/ijsem.0.004530.
A Gram-staining-negative, aerobic, cream-coloured, marine bacterium, with rod-shaped cells, designated strain YJ-S3-2, was isolated from salt flat sediment of Yongyu-do, Republic of Korea. YJ-S3-2 grew at pH 5.0-9.0 (optimum pH 7.0), 4-45 °C (optimum 30 °C) and with 1-18 % (w/v) NaCl (optimum 6 %). The results of 16S rRNA gene sequence analysis indicated that YJ-S3-2 was closely related to SS011B1-4 (97.0 %) followed by, '' D15-8W (96.7 %), 50-11 (96.7 %), DSMZ 179240 (96.5 %) and T17 (96.5 %). The average nucleotide identity (ANI) and the genome to genome distance calculator (GGDC) estimate values between YJ-S3-2 and related type strains were 73.7-79.8 and 19.9-22.5 %, and also 73.5 and 20.7 % with . YJ-S3-2 was characterized as having Q-9 as the predominant respiratory quinone and the principal fatty acids (>10 %) were C (22.3 %), summed feature 9 (Ciso ω9/C 10-methyl, 13.8 %) and 3 (Cω7C1ω6 11.9 %). The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, two unidentified aminolipids and two unidentified phospholipids. The DNA G+C content of YJ-S3-2 is 60.9 mol%. On the basis of the polyphasic taxonomic evidence presented in this study, YJ-S3-2 should be classified as representing a novel species within the genus , for which name sp. nov. is proposed, with the type strain YJ-S3-2 (=KACC 19883=KCTC 62937=JCM 33109).
一株革兰氏阴性、需氧、奶油色的海洋细菌，细胞呈杆状，被命名为 YJ-S3-2 菌株，从韩国永宇岛盐滩沉积物中分离得到。YJ-S3-2 在 pH 值为 5.0-9.0（最佳 pH 值为 7.0）、4-45°C（最佳温度为 30°C）和 1-18%（w/v）NaCl（最佳浓度为 6%）的条件下生长。16S rRNA 基因序列分析结果表明，YJ-S3-2 与 SS011B1-4（97.0%）密切相关，其次是 D15-8W（96.7%）、50-11（96.7%）、DSMZ 179240（96.5%）和 T17（96.5%）。YJ-S3-2 与相关模式菌株的平均核苷酸同一性（ANI）和基因组到基因组距离计算器（GGDC）估计值分别为 73.7-79.8%和 19.9-22.5%，与. 的 ANI 和 GGDC 估计值分别为 73.5%和 20.7%。YJ-S3-2 的主要呼吸醌为 Q-9，主要脂肪酸（>10%）为 C（22.3%）、summed feature 9（Ciso ω9/C 10-methyl，13.8%）和 3（Cω7C1ω6，11.9%）。极性脂由双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、两种未鉴定的胺脂和两种未鉴定的磷脂组成。YJ-S3-2 的 DNA G+C 含量为 60.9mol%。基于本研究中提供的多相分类证据，YJ-S3-2 应归类为属于属内的一个新种，建议使用新种名 sp. nov.，其模式菌株为 YJ-S3-2（=KACC 19883=KCTC 62937=JCM 33109）。
