Center of Excellence for Infection Control Science, Graduate School of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
School of Medicine, Juntendo University, 2-1-1 Hongo, Bunkyo-ku, Tokyo, 113-8421, Japan.
Curr Microbiol. 2020 Aug;77(8):1909-1915. doi: 10.1007/s00284-020-01943-2. Epub 2020 Mar 18.
During the exploration of microbial natural resources, two strains of Pseudomonas, PS14 and PS24, were isolated from samples taken from Izu Oshima, a volcanic island located 120 km southwest of central Tokyo. Phylogenetic analysis based on 16S rRNA gene sequences showed that PS14 was most similar to Pseudomonas baetica a390 (99.6%) and Pseudomonas helmanticensis OHA11 (99.5%), and that PS24 was most similar to Pseudomonas qingdaonensis JJ3 (98.8%) and Pseudomonas lutea OK2 (98.7%). The major fatty acids of these two strains were C and C cyclo, summed feature 3 (C ω6c and/or C ω7c), and summed feature 8 (C ω7c and/or ω6c). The phylogenetic analyses, DNA-DNA hybridization results and phenotypic traits indicated that PS14 and PS24 constitute two novel species, Pseudomonas atagosis sp. nov. (type strain PS14 = CECT 9940, = LMG 31496) and Pseudomonas akappagea sp. nov. (type strain PS24 = CECT 9941, = LMG 31497), respectively. The sequence data of the draft genomes of PS14 and PS24 were deposited in the GenBank database under accession numbers VXCA00000000 and VXCP00000000, respectively, and the sequence data of their 16S rRNA genes were deposited in the GenBank database under accession numbers MN396717 and MN382268, respectively.
在探索微生物自然资源的过程中，从位于东京市中心西南 120 公里的伊豆小笠原群岛采集的样本中分离出了两株假单胞菌，PS14 和 PS24。基于 16S rRNA 基因序列的系统发育分析表明，PS14 与 Pseudomonas baetica a390（99.6%）和 Pseudomonas helmanticensis OHA11（99.5%）最为相似，PS24 与 Pseudomonas qingdaonensis JJ3（98.8%）和 Pseudomonas lutea OK2（98.7%）最为相似。这两株菌的主要脂肪酸为 C 和 C cyclo、summed feature 3（C ω6c 和/或 C ω7c）和 summed feature 8（C ω7c 和/或 ω6c）。系统发育分析、DNA-DNA 杂交结果和表型特征表明，PS14 和 PS24 构成了两个新种，即 Atagosis pseudomonas sp. nov.（模式菌株 PS14=CECT 9940= LMG 31496）和 Akappagea pseudomonas sp. nov.（模式菌株 PS24=CECT 9941=LMG 31497）。PS14 和 PS24 的基因组草图序列数据分别保存在 GenBank 数据库中，登录号为 VXCA00000000 和 VXCP00000000，16S rRNA 基因序列数据分别保存在 GenBank 数据库中，登录号为 MN396717 和 MN382268。
