Bell E B, Shand F L
Immunology. 1977 Oct;33(4):469-76.
Thoracic duct lymphocytes (TDL) from (AS2 x AS)F1 rats previously injected with human serum albumin (HSA) were transferred to 900 r irradiated syngeneic recipients which were challenged with various doses of soluble HSA (s-HSA). TDL from partially tolerant rats, which were deficient in high avidity B cells, produced a maximum PFC response to the largest challenge dose (1 g s-HSA). In contrast, high avidity B cells from primed donors were optimally stimulated by 1 microgram and maximally inhibited by 1 mg s-HSA (day 7 PFC). An additional increase in antigen concentration by 1000 fold failed to diminish the PFC numbers further. Plaque inhibition profiles indicated that these antibody forming cells resisting inhibition were of the same high avidity as those triggered by low doses of antigen. The inability of s-HSA to completely inhibit antibody synthesis is discussed with regard to current views on B cell inactivation. Evidence is also presented which indicates that the standard haemolysis-in-gel test may fail to detect many low avidity antibody forming cells to proteins.
将先前注射过人血清白蛋白（HSA）的（AS2×AS）F1大鼠的胸导管淋巴细胞（TDL）转移至经900伦琴照射的同基因受体，这些受体用不同剂量的可溶性HSA（s-HSA）进行攻击。来自部分耐受大鼠的TDL缺乏高亲和力B细胞，对最大攻击剂量（1克s-HSA）产生最大的空斑形成细胞（PFC）反应。相比之下，来自致敏供体的高亲和力B细胞在1微克s-HSA刺激下达到最佳状态，在1毫克s-HSA时受到最大抑制（第7天的PFC）。抗原浓度再增加1000倍也未能进一步减少PFC数量。空斑抑制图谱表明，这些抵抗抑制的抗体形成细胞与低剂量抗原触发的细胞具有相同的高亲和力。关于B细胞失活的当前观点，讨论了s-HSA无法完全抑制抗体合成的情况。还提供了证据表明，标准的凝胶内溶血试验可能无法检测到许多针对蛋白质的低亲和力抗体形成细胞。
