体外抗体介导的免疫反应抑制。II. 免疫耐受现象的一种新方法。
Antibody-mediated suppression of the immune response in vitro. II. A new approach to the phenomenon of immunological tolerance.
作者信息
Diener E, Feldmann M
出版信息
J Exp Med. 1970 Jul 1;132(1):31-43. doi: 10.1084/jem.132.1.31.
Immunological tolerance to H antigens of Salmonella adelaide may be induced in vitro by the exposure of mouse spleen cells for 6 hr to an immunogenic dose of polymerized flagellin in the presence of low concentrations of specific antibody. Such antibody-mediated tolerance requires an optimal antigen: antibody ratio for its induction. A shift in this ratio in favor of the antibody concentration results in failure of tolerance induction and leads to immune suppression commonly known as antibody-mediated feedback inhibition which is not analogous to immunological tolerance. Fragment A of flagellin fails to induce immunological tolerance in vitro. Tolerance to polymerized flagellin may however be induced in vitro, provided the spleen cells are exposed to fragment A in the presence of specific antibody for 6 hr. The results are discussed in the light of current theories of the mechanism of tolerance induction.
在低浓度特异性抗体存在的情况下，将小鼠脾细胞暴露于免疫原剂量的聚合鞭毛蛋白6小时，可在体外诱导对阿德莱德沙门氏菌H抗原的免疫耐受。这种抗体介导的耐受诱导需要最佳的抗原：抗体比例。该比例向有利于抗体浓度的方向转变会导致耐受诱导失败，并导致通常称为抗体介导的反馈抑制的免疫抑制，这与免疫耐受不同。鞭毛蛋白的A片段在体外不能诱导免疫耐受。然而，如果脾细胞在特异性抗体存在的情况下暴露于A片段6小时，则可在体外诱导对聚合鞭毛蛋白的耐受。根据目前关于耐受诱导机制的理论对结果进行了讨论。
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