新型共识细菌 6-植酸酶变体完全替代了肉鸡日粮中的无机磷，保持了生长性能和骨骼质量：来自两项独立试验的数据。
A novel consensus bacterial 6-phytase variant completely replaced inorganic phosphate in broiler diets, maintaining growth performance and bone quality: data from two independent trials.
机构信息
Animal Nutrition, DuPont Nutrition and Biosciences, 2342 BH Oegstgeest, the Netherlands; Animal Nutrition Group, Wageningen University & Research, Wageningen, the Netherlands.
Animal Nutrition, DuPont Nutrition and Biosciences, 2342 BH Oegstgeest, the Netherlands.
出版信息
Poult Sci. 2021 Mar;100(3):100962. doi: 10.1016/j.psj.2020.12.059. Epub 2020 Dec 24.
Total replacement of dietary inorganic phosphate (Pi) by a novel consensus bacterial 6-phytase variant (PhyG) in phytate-rich diets (>0.3% phytate-P) was investigated in 2 trials using growth performance and bone quality as outcome measures. Both trials utilized a completely randomized design with 5 dietary treatments across 4 phases: starter (0-10 d), grower (10-21 d), finisher 1 (21-35 d), and finisher 2 (35-42 d). Treatments comprised a nutritionally adequate positive control (PC) diet containing monocalcium phosphate and 4 experimental diets (IPF1, IPF2, IPF3, and IPF4), all containing no added Pi and reduced in Ca by 0.2 to 0.3% units vs. PC. IPF1contained PhyG at 1,000 FTU/kg (all phases); IPF2 contained PhyG at 1,000 FTU/kg (all phases) and was additionally reduced in digestible AA, ME, and sodium (-0.2 to -0.4% points, -74 kcal/kg, -0.04% points, respectively, vs. PC); IPF3 contained PhyG at 3,000 FTU/kg in starter, 2,000 FTU/kg in grower, and 1,000 FTU/kg in finisher phases; and IPF4 contained xylanase (2,000 U/kg) and PhyG (2,000 FTU/kg in starter, 1,500 FTU/kg in grower, and 1,000 FTU/kg in finisher phases) and was additionally reduced in ME (-71 kcal/kg vs. PC). Ross 308 broilers were used (trial 1: n = 1,200 mixed sex; 24 birds per pen × 10 replicates; trial 2: n = 1,300 males; 26 birds × 10 replicates). During all phases in both trials, all IPF treatments maintained or improved BW, ADG, ADFI, FCR and BW-corrected FCRc and bone quality parameters vs. PC. vs. PC, treatment IPF3 increased ADG during starter phase (+10.8%) and reduced overall FCRc (-12 points, P < 0.05) in Trial 1, and increased overall ADG (+4.4%), day 35 and day 42 BW (+3.5%, +4.9%), and reduced overall FCRc (-11 points) in Trial 2 (P < 0.05). IPF4 produced equivalent performance to IPF3 (both trials). These are the first data to demonstrate total replacement of Pi by microbial phytase during an entire growth cycle in broiler diets.
在两项试验中，使用生长性能和骨骼质量作为结果测量，研究了在富含植酸（植酸盐-P 含量>0.3%）的日粮中，用新型共识细菌 6-植酸酶变体（PhyG）完全替代日粮中的无机磷酸盐（Pi）。这两项试验均采用完全随机设计，有 4 个阶段的 5 种日粮处理：起始期（0-10d）、生长期（10-21d）、育肥期 1（21-35d）和育肥期 2（35-42d）。处理组包括一个营养充足的阳性对照（PC）日粮，含有磷酸一钙和 4 种试验日粮（IPF1、IPF2、IPF3 和 IPF4），均不含添加的 Pi，与 PC 相比，Ca 降低了 0.2 到 0.3 个单位。IPF1 在所有阶段均含有 1000FTU/kg 的 PhyG；IPF2 在所有阶段均含有 1000FTU/kg 的 PhyG，并且可消化氨基酸、代谢能和钠的含量分别降低了 0.2 到 0.4 个百分点（-74kcal/kg）、0.04 个百分点（-0.04%）和-0.4%（与 PC 相比）；IPF3 在起始期含有 3000FTU/kg 的 PhyG、生长期含有 2000FTU/kg 的 PhyG 和育肥期含有 1000FTU/kg 的 PhyG；IPF4 含有木聚糖酶（2000U/kg）和 2000FTU/kg 的 PhyG（在起始期、生长期和育肥期分别为 1500FTU/kg 和 1000FTU/kg），代谢能降低了 71kcal/kg（与 PC 相比）。试验使用了罗斯 308 肉鸡（试验 1：n=1200 只，雌雄混合；每栏 24 只鸡×10 个重复；试验 2：n=1300 只公鸡；每栏 26 只鸡×10 个重复）。在这两项试验的所有阶段，所有的 IPF 处理组与 PC 相比，均维持或提高了 BW、ADG、ADFI、FCR 和 BW 校正 FCRc 以及骨骼质量参数。与 PC 相比，试验 1 中的处理组 IPF3 在起始期提高了 ADG（+10.8%），降低了总 FCRc（-12 点，P<0.05），在试验 2 中提高了总 ADG（+4.4%）、第 35 天和第 42 天 BW（+3.5%，+4.9%），并降低了总 FCRc（-11 点，P<0.05）。IPF4 的性能与 IPF3 相当（两个试验）。这些数据首次表明，在肉鸡日粮的整个生长周期中，微生物植酸酶可以完全替代 Pi。
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