Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, Germany.
Institute of Epidemiology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, Germany.
Viruses. 2021 Feb 26;13(3):370. doi: 10.3390/v13030370.
Arthropod-borne Batai virus (BATV) is an widely distributed throughout European livestock and has, in the past, been linked to febrile diseases in humans. In Germany, BATV was found in mosquitoes and in one captive harbor seal, and antibodies were recently detected in various ruminant species. We have, therefore, conducted a follow-up study in ruminants from Saxony-Anhalt, the most affected region in Eastern Germany. A total of 325 blood samples from apparently healthy sheep, goats, and cattle were tested using a BATV-specific qRT-PCR and SNT. Even though viral RNA was not detected, the presence of antibodies was confirmed in the sera of all three species: sheep (16.5%), goats (18.3%), and cattle (41.4%). Sera were further analyzed by a glycoprotein Gc-based indirect ELISA to evaluate Gc-derived antibodies as a basis for a new serological test for BATV infections. Interestingly, the presence of neutralizing antibodies was not directly linked to the presence of BATV Gc antibodies. Overall, our results illustrate the high frequency of BATV infections in ruminants in Eastern Germany.
虫媒乙型脑炎病毒（BATV）广泛分布于欧洲的牲畜中，过去曾与人类的发热疾病有关。在德国，BATV 在蚊子和一只圈养的海豹中被发现，最近在各种反刍动物物种中检测到了抗体。因此，我们在德国东部受影响最严重的萨克森-安哈尔特州的反刍动物中进行了一项后续研究。使用 BATV 特异性 qRT-PCR 和 SNT 检测了来自健康绵羊、山羊和牛的总共 325 份血液样本。尽管未检测到病毒 RNA，但在三种动物的血清中均证实存在抗体：绵羊（16.5%）、山羊（18.3%）和牛（41.4%）。血清进一步通过基于糖蛋白 Gc 的间接 ELISA 进行分析，以评估 Gc 衍生抗体作为 BATV 感染新血清学检测的基础。有趣的是，中和抗体的存在与 BATV Gc 抗体的存在没有直接联系。总体而言，我们的研究结果说明了德国东部反刍动物中 BATV 感染的高频率。
