Mondragón-Rodríguez Siddhartha, Ordaz Benito, Orta-Salazar Erika, Díaz-Cintra Sofia, Peña-Ortega Fernando, Perry George
National Council for Science and Technology (CONACYT), México, México.
Developmental Neurobiology and Neurophysiology, Institute of Neurobiology, National Autonomous University of México (UNAM), Querétaro, México.
Bio Protoc. 2020 Feb 20;10(4):e3529. doi: 10.21769/BioProtoc.3529.
Transgenic mice have been used to make valuable contributions to the field of neuroscience and model neurological diseases. The simultaneous functional analysis of hippocampal cell activity combined with hippocampal dependent innate task evaluations provides a reliable experimental approach to detect fine changes during early phases of neurodegeneration. To this aim, we used a merge of patch-clamp with two hippocampal innate behavior tasks. With this experimental approach, whole-cell recordings of CA1 pyramidal cells, combined with hippocampal-dependent innate behaviors, have been crucial for evaluating the early mechanism of neurodegeneration and its consequences. Here, we present our protocol for whole-cell recordings of CA1 pyramidal cells and hippocampal dependent innate behaviors in an adolescent (p30) mice.
转基因小鼠已为神经科学领域和神经疾病模型做出了重要贡献。海马体细胞活动的同步功能分析与海马体依赖的先天性任务评估相结合，为检测神经退行性变早期阶段的细微变化提供了一种可靠的实验方法。为此，我们将膜片钳技术与两项海马体先天性行为任务相结合。通过这种实验方法，CA1锥体神经元的全细胞记录与海马体依赖的先天性行为相结合，对于评估神经退行性变的早期机制及其后果至关重要。在此，我们展示了在青春期（p30）小鼠中进行CA1锥体神经元全细胞记录和海马体依赖的先天性行为的实验方案。
