Massalha Hassan, Korenblum Elisa, Shapiro Orr H, Aharoni Asaph
Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot 76100, Israel.
Department of Food Sciences, Institute for Postharvest and Food Sciences, Agricultural Research Organization, The Volcani Center, Rishon LeZion 7528809, Israel.
Bio Protoc. 2019 Apr 20;9(8):e3211. doi: 10.21769/BioProtoc.3211.
Soil organisms are diverse taxonomically and functionally. This ecosystem experiences highly complex networks of interactions, but may also present functionally independent entities. Plant roots, a metabolically active hotspot in the soil, take an essential part in shaping the rhizosphere. Tracking the dynamics of root-microbe interactions at high spatial resolution is currently limited due to methodological intricacy. In this study, we developed a novel microfluidics-based device enabling direct imaging of root-bacteria interactions in real time.
土壤生物在分类学和功能上具有多样性。这个生态系统经历着高度复杂的相互作用网络，但也可能呈现出功能上独立的实体。植物根系是土壤中一个代谢活跃的热点，在塑造根际方面起着至关重要的作用。由于方法上的复杂性，目前在高空间分辨率下追踪根与微生物相互作用的动态变化受到限制。在本研究中，我们开发了一种基于微流控的新型装置，能够实时直接成像根与细菌的相互作用。
