Zou Yang, Long Saran, Xiong Tao, Zhao Xueze, Sun Wen, Du Jianjun, Fan Jiangli, Peng Xiaojun
State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024, China.
Ningbo Institute of Dalian University of Technology, Ningbo 315016, China.
ACS Cent Sci. 2021 Feb 24;7(2):327-334. doi: 10.1021/acscentsci.0c01551. Epub 2021 Jan 22.
Photosensitizers (PSs) inevitably release a large amount of energy in the form of fluorescence during photodynamic therapy (PDT). However, under the premise of satisfying fluorescence imaging, a large amount of energy is lost, which limits the efficiency of tumor therapy. Accordingly, in this study, we developed a new strategy () using the single-molecule Förster resonance energy transfer (smFRET) mechanism to transfer part of the fluorescent energy into heat for combined PDT and photothermal therapy (PTT) featuring the "1 + 1 > 2" amplification effect. Under the 671 nm light irradiation, can produce singlet oxygen (O) for PDT based on the moiety and also generate hyperthermia to achieve the PTT effect by exciting based on the smFRET effect, which effectively kills cancer cells both and . This strategy exhibits a broad absorption peak with strong light-harvesting ability, which improves photon utilization for treatment while realizing fluorescence imaging. Of note, owing to the smFRET effect, we achieve a combination treatment outcome at relatively low concentrations and light doses. Thus, we believe that this design concept will provide a new strategy for single-molecule FRET photosensitizers in combination therapy of cancer with potential clinical application prospects.
在光动力疗法（PDT）过程中，光敏剂（PSs）不可避免地会以荧光的形式释放大量能量。然而，在满足荧光成像的前提下，大量能量会损失，这限制了肿瘤治疗的效率。因此，在本研究中，我们开发了一种新策略（），利用单分子Förster共振能量转移（smFRET）机制将部分荧光能量转化为热量，用于联合光动力疗法和光热疗法（PTT），具有“1 + 1 > 2”的放大效应。在671 nm光照射下，基于部分可产生单线态氧（O）用于光动力疗法，同时基于smFRET效应激发产生热以实现光热疗法效果，这有效地从两方面杀死癌细胞。该策略展现出具有强光捕获能力的宽吸收峰，在实现荧光成像的同时提高了治疗的光子利用率。值得注意的是，由于smFRET效应，我们在相对较低的浓度和光剂量下实现了联合治疗效果。因此，我们相信这种设计理念将为单分子FRET光敏剂在癌症联合治疗中提供一种具有潜在临床应用前景的新策略。
