Department of Urology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China.
Mol Med Rep. 2021 Mar;23(3). doi: 10.3892/mmr.2020.11819. Epub 2020 Dec 31.
The formation and physicochemical properties of kidney stones (KSs) are closely associated with diet. In view of the differences in ethnicity and dietary composition between Chinese and Western populations, the present study aimed to investigate the association between intestinal dysbacteriosis and KSs in China. The current study examined the differences in intestinal microbes between the KS disease (KSD) and the healthy control (HLT) groups, and statistically significant differences based on 16s rRNA gene amplicons were identified using a Student's t‑test or one‑way ANOVA. In addition, the calcium oxalate KS (COKS), uric acid KS (UAKS) and carbonate apatite KS(CCKS) groups were compared with a non‑parametric statistical test. Determination of bacterial abundance was performed via the analysis of 16s rRNA marker gene sequences using next‑generation sequencing. Firmicutes (F) and Bacteroides (B) levels were significantly higher in the KSD group compared with the HLT group (B/F=0.67 vs. 0.08; P<0.001), as were the overall levels of B (6.19‑fold higher compared with the HLT group; 22.2 vs. 3.6%; P<0.001). The Prevotella‑9 abundance levels in the KSD group were 4.65‑fold higher compared with those in the HLT group (8.8 vs. 1.9%; P<0.001). The levels of Blautia and Lachnoclostridium were significantly decreased in the KSD group (13.3 vs. 6.0%; and 5.0 vs. 7.9%; both P<0.05). Moreover, Prevotella‑9 levels were higher in non‑calciferous KSs (UAKS) compared with calciferous KSs (COKS and CCKS). Therefore, the findings of the present study indicated a key association between specific KS components and intestinal flora, providing a theoretical basis for new treatment methods for KSs. Moreover, differences and interactions between these bacteria could initially predict specific types of urolithiasis.
肾结石（KSs）的形成和理化性质与饮食密切相关。鉴于中西方人群在种族和饮食组成上存在差异，本研究旨在探讨中国肠菌失调与 KSs 之间的关系。本研究检测了 KS 疾病（KSD）与健康对照组（HLT）之间肠道微生物的差异，并通过学生 t 检验或单向方差分析比较了基于 16s rRNA 基因扩增子的统计学差异。此外，还对草酸钙 KS（COKS）、尿酸 KS（UAKS）和碳酸磷灰石 KS（CCKS）组进行了非参数统计检验。通过下一代测序分析 16s rRNA 标记基因序列来确定细菌丰度。Firmicutes（F）和Bacteroides（B）在 KSD 组中的水平明显高于 HLT 组（B/F=0.67 比 0.08；P<0.001），B 的总体水平也明显高于 HLT 组（高 6.19 倍；22.2 比 3.6%；P<0.001）。KSD 组中Prevotella-9的丰度水平比 HLT 组高 4.65 倍（8.8 比 1.9%；P<0.001）。KSD 组中Blautia和Lachnoclostridium的水平明显降低（13.3 比 6.0%；和 5.0 比 7.9%；均 P<0.05）。此外，Prevotella-9的水平在非钙性 KSs（UAKS）中高于钙性 KSs（COKS 和 CCKS）。因此，本研究的结果表明，特定 KS 成分与肠道菌群之间存在重要关联，为 KS 新的治疗方法提供了理论依据。此外，这些细菌之间的差异和相互作用可以初步预测特定类型的尿石症。
