人多能干细胞衍生的背根脊感觉神经元。

Department of Neurobiology, University of California Los Angeles, Los Angeles, CA 90095, USA.

Eli & Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California Los Angeles, Los Angeles, CA 90095, USA.

STAR Protoc. 2021 Feb 3;2(1):100319. doi: 10.1016/j.xpro.2021.100319. eCollection 2021 Mar 19.

We describe two differentiation protocols to derive sensory spinal interneurons (INs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). In protocol 1, we use retinoic acid (RA) to induce pain, itch, and heat mediating dI4/dI6 interneurons, and in protocol 2, RA with bone morphogenetic protein 4 (RA+BMP4) is used to induce proprioceptive dI1s and mechanosensory dI3s in hPSC cultures. These protocols provide an important step toward developing therapies for regaining sensation in spinal cord injury patients. For complete details on the use and execution of this protocol, please refer to Gupta et al. (2018).

我们描述了两种从人胚胎干细胞（hESC）和诱导多能干细胞（iPSC）中分化感觉性脊髓中间神经元（IN）的方法。在方案 1 中，我们使用视黄酸（RA）诱导痛觉、痒觉和热觉介导的 dI4/dI6 中间神经元，在方案 2 中，RA 与骨形态发生蛋白 4（RA+BMP4）一起诱导 hPSC 培养物中的本体感觉性 dI1s 和机械感觉性 dI3s。这些方案为开发治疗脊髓损伤患者感觉恢复的方法提供了重要步骤。有关此方案使用和执行的完整详细信息，请参阅 Gupta 等人。（2018 年）。