Faculty of Technology, University of Novi Sad, Bulevar cara Lazara 1, 21000, Novi Sad, Serbia.
Institute of Food Technology, University of Novi Sad, Bulevar cara Lazara 1, 21000, Novi Sad, Serbia.
Chem Biodivers. 2021 Apr;18(4):e2100058. doi: 10.1002/cbdv.202100058. Epub 2021 Mar 9.
The aim of this study was to extract Allium ursinum L. for the first time by supercritical carbon dioxide (SC-CO ) as green sustainable method. The impact of temperature in the range from 40 to 60 °C and pressure between 150 and 400 bar on the quality of the obtained extracts and efficiency of the extraction was investigated. The highest extraction yield (3.43 %) was achieved by applying the extraction conditions of 400 bar and 60 °C. The analysis of the extracts was performed by gas chromatography and mass spectrometry (GC/MS). The most dominant sulfur-containing constituent of the extracts was allyl methyl trisulfide with the highest abundance at 350 bar and 50 °C. In addition, the presence of other pharmacologically potent sulfur compounds was recorded including S-methyl methanethiosulfinate, diallyl trisulfide, S-methyl methylthiosulfonate, and dimethyl trisulfide. Multivariate data analysis tool was utilized to investigate distributions of the identified compounds among the extracts obtained under various extraction conditions and yields. It was determined that the SC-CO extraction can by efficiently used for A. ursinum.
本研究旨在首次通过超临界二氧化碳（SC-CO ）萃取来提取熊葱。考察了温度在 40 至 60°C 范围内和压力在 150 至 400 巴对所得提取物的质量和萃取效率的影响。通过应用 400 巴和 60°C 的萃取条件，获得了最高的萃取产率（3.43%）。通过气相色谱和质谱分析（GC/MS）对提取物进行了分析。提取物中含量最高的含硫成分是烯丙基甲基三硫化物，在 350 巴和 50°C 时含量最高。此外，还记录了其他具有药理活性的含硫化合物的存在，包括 S-甲基甲硫亚砜、二烯丙基三硫化物、S-甲基甲基硫代亚砜和二甲基三硫化物。利用多元数据分析工具研究了在各种萃取条件下获得的提取物中鉴定化合物的分布和产率。结果表明，SC-CO 萃取可有效地用于熊葱。
