Department of Medical Biotechnology, Biomedical Campus 32, Gyeonggi 10326, Republic of Korea.
School of Chemical Engineering, Yeungnam University, 280 Daehak-Ro, Gyeongsan 38541, Republic of Korea.
Int J Biol Macromol. 2021 May 1;178:464-476. doi: 10.1016/j.ijbiomac.2021.02.195. Epub 2021 Mar 1.
Polymer-clay nanocomposite hydrogel films (PCNCHFs) were prepared from caboxymethyl cellulose, polyvinylpyrrolidone, agar and nanosepiolite clay (0, 0.3, 0.5, 0.7, 0.9 and 1.5% reinforcement) by treating thermally in a simple, rapid, and inexpensive route. The PCNCHFs and its 5-fluorouracil (FU)-loaded composites (PCNCHFs@FU) were tested for FU release and characterized by FTIR, XRD, FE-SEM, EDX, DSC, and TGA analyses to investigate their structural, morphological, and thermal properties. The nanosepiolite-loaded polymer composites (PCNCHF1 to PCNCHF5) exhibited higher tensile strength than the pristine polymer hydrogel (PCNCHF0); consequently, the thermal properties (glass- and melting-transition) were improved. The PCNCHFs@FU demonstrated prolonged FU release at pH 7.4 for 32 h. The biocompatibility of PCNCHFs was tested against human skin fibroblast (CCDK) cells. The viability of cells exposed to all PCNCHFs was >95% after 72 h of culture. The live/dead assay show the proliferation of fibroblast cells, confirming the biocompatibility of the hydrogels. The pH-sensitive PCNCHFs@FU release could be suitable for drug release in cancer therapy, and the developed PCNCHFs may also be useful for tissue engineering, food packaging, and other biological applications.
聚合物-粘土纳米复合水凝胶薄膜（PCNCHF）由羧甲基纤维素、聚乙烯吡咯烷酮、琼脂和纳米海泡石粘土（0、0.3、0.5、0.7、0.9 和 1.5%增强剂）制备，通过在简单、快速且廉价的路线中进行热处理。PCNCHF 和其负载 5-氟尿嘧啶（FU）的复合材料（PCNCHF@FU）进行 FU 释放测试，并通过 FTIR、XRD、FE-SEM、EDX、DSC 和 TGA 分析进行结构、形态和热性能表征。负载纳米海泡石的聚合物复合材料（PCNCHF1 至 PCNCHF5）表现出比原始聚合物水凝胶（PCNCHF0）更高的拉伸强度；因此，热性能（玻璃化转变和熔融转变）得到改善。PCNCHF@FU 在 pH 7.4 下显示出长达 32 小时的 FU 释放延长。PCNCHF 的生物相容性通过人皮肤成纤维细胞（CCDK）细胞进行测试。暴露于所有 PCNCHF 的细胞在培养 72 小时后存活率>95%。活/死测定显示成纤维细胞的增殖，证实了水凝胶的生物相容性。pH 敏感的 PCNCHF@FU 释放可能适用于癌症治疗中的药物释放，并且开发的 PCNCHF 也可能用于组织工程、食品包装和其他生物应用。
