Wardani Indah Sapta, Hatta Mochammad, Mubin Risna Halim, Bukhari Agussalim, Massi Muhammad Nasrum, Djaharuddin Irawaty, Bahar Burhanuddin, Wahyuni Siti
Post Graduate School, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia.
Department of Internal Medicine, Faculty of Medicine, Mataram University, Mataram, Indonesia.
Ann Med Surg (Lond). 2021 Feb 12;63:102174. doi: 10.1016/j.amsu.2021.02.020. eCollection 2021 Mar.
HIV-AIDS patients typically have hypovitaminosis D. Vitamin D is a key mediator in inflammatory and infectious diseases, which VDR mediates its biological effect. High-mobility group box 1 protein (HMGB1) modulates HIV-1 replication in vitro. Vitamin D played a role in inhibiting HMGB1 secretion in the animal study.
This study aimed to examine differences and correlation of vitamin D receptor and HMGB1 protein levels in HIV patients with mild and severe immunodeficiency and healthy control participants.
This study using a cross-sectional design conducted at Volunteer Counseling and Testing (VCT) Clinic in Mataram, West Nusa Tenggara, Indonesia, from January to June 2020. Three groups of study participants were classified as HIV patients with severe immune deficiency (SID), HIV patients with mild immune deficiency (MID), and healthy controls (HC).
Mean level of vitamin D receptor in SID HIV group was 25.89 ± 3.95 ng/ml, lower than those in MID-HIV group; 33.72 ± 1.69 ng/ml and in HC group; 50.65 ± 3.64 ng/ml. Mean levels of HMGB1 protein in the SID HIV group were 3119.81 ± 292.38 pg/ml higher than those in the MID HIV group 1553.55 ± 231.08 pg/ml and HC 680.82 ± 365.51 pg/ml. There was a significant and strong negative correlation (r = -0.932) between vitamin D receptor and HMGB1 levels ( < 0.01).
Strong negative correlation between VDR and HMGB1 in different immunodeficiency statuses suggesting an important role of vitamin D in inflammation control in HIV infection. However, it needs to be confirmed in a further prospective study.
艾滋病患者通常存在维生素D缺乏症。维生素D是炎症和感染性疾病中的关键介质，维生素D受体介导其生物学效应。高迁移率族蛋白B1（HMGB1）在体外调节HIV-1复制。在动物研究中，维生素D在抑制HMGB1分泌方面发挥了作用。
本研究旨在探讨免疫缺陷程度不同的艾滋病患者与健康对照者之间维生素D受体和HMGB1蛋白水平的差异及相关性。
本研究采用横断面设计，于2020年1月至6月在印度尼西亚西努沙登加拉省马塔兰的自愿咨询检测（VCT）诊所进行。三组研究参与者分别被分类为严重免疫缺陷（SID）的艾滋病患者、轻度免疫缺陷（MID）的艾滋病患者和健康对照者（HC）。
SID艾滋病组维生素D受体的平均水平为25.89±3.95 ng/ml，低于MID艾滋病组（33.72±1.69 ng/ml）和HC组（50.65±3.64 ng/ml）。SID艾滋病组HMGB1蛋白的平均水平为3119.81±292.38 pg/ml，高于MID艾滋病组（1553.55±231.08 pg/ml）和HC组（680.82±365.51 pg/ml）。维生素D受体与HMGB1水平之间存在显著且强烈的负相关（r = -0.932，P < 0.01）。
不同免疫缺陷状态下VDR与HMGB1之间存在强烈的负相关，提示维生素D在控制HIV感染炎症方面具有重要作用。然而，这需要在进一步的前瞻性研究中得到证实。
