Akaputra Risky, Hatta Mochammad, Massi Muhammad N, Djaharuddin Irawati, Bukhari Agussalim, Aminuddin Aminuddin, Santoso Arif, Natzir Rosdiana, Bahar Burhanudin, Fachri Muhammad, Farsida Farsida, Fathimah Agus, Ubaidah Firda M, Sridiana Eva, Dwiyanti Ressy, Syukri Ahmad, Junita Ade R, Febrianti Andini, Primaguna Muhammad R, Azhar Azhar
Department of Pulmonology and Respiratory Medicine, Faculty of Medicine and Health, Universitas Muhammadiyah Jakarta, Jakarta.
Department of Molecular Biology and Immunology.
Ann Med Surg (Lond). 2023 May 23;85(6):2395-2399. doi: 10.1097/MS9.0000000000000908. eCollection 2023 Jun.
Pneumonia is one of the most common infections caused by the bacterium . During the initiation of an infection, the immune system recognizes the pathogen through the release of high mobility group box 1 (HMGB1), thereby triggering the inflammation process. Miana has demonstrated potent inhibitory effects on the inflammatory process during infection in animal models. The aim of this study was to determine the effect of Miana leaf extract on mRNA HMGB1 expression in Balb/c mice infected with .
This study comprised a cohort experiment using 20 Balb/c mice divided into four groups. Balb/c mice in each group were intraperitoneally injected with . Group 1 was given a placebo; Group 2 was given Miana; Group 3 was given levofloxacin; and Group 4 was given both levofloxacin and Miana. The levels of mRNA HMGB1 expression were measured using real-time PCR before, during, and after the infection as well as after the treatments.
The initial examination results showed that the average level of mRNA HMGB1 expression was 5.51 fc. The mRNA HMGB1 expression in mice after being challenged with was 9.64 fc. Group 1 that was given a placebo had a mean mRNA HMGB1 expression level of 14.99 fc. Group 2 that was given Miana had a mean mRNA HMGB1 expression level of 13.95 fc. Group 3 that was given levofloxacin had an average mRNA HMGB1 expression level of 6.45 fc, and Group 4 that was given levofloxacin and Miana together had an average mRNA HMGB1 expression level of 5.59 fc.
Miana ( (L.) Benth) increased mRNA HMGB1 expression at the initial administration via regulation of the immune system. Administration of Miana following infection inhibited the increase in mRNA HMGB1 expression. Treatment with levofloxacin reduced the level of mRNA HMGB1 expression, and the effect was optimized by the administration of Miana leaf extract as a supplement.
肺炎是由该细菌引起的最常见感染之一。在感染初期，免疫系统通过释放高迁移率族蛋白B1（HMGB1）识别病原体，从而引发炎症过程。Miana在动物模型感染期间对炎症过程表现出强大的抑制作用。本研究的目的是确定Miana叶提取物对感染[细菌名称未给出]的Balb/c小鼠中HMGB1 mRNA表达的影响。
本研究采用队列实验，将20只Balb/c小鼠分为四组。每组Balb/c小鼠腹腔注射[细菌名称未给出]。第1组给予安慰剂；第2组给予Miana；第3组给予左氧氟沙星；第4组给予左氧氟沙星和Miana。在感染前、感染期间、感染后以及治疗后，使用实时PCR测量HMGB1 mRNA表达水平。
初始检查结果显示，HMGB1 mRNA表达的平均水平为5.51倍变化（fc）。用[细菌名称未给出]攻击后小鼠的HMGB1 mRNA表达为9.64 fc。给予安慰剂的第1组HMGB1 mRNA表达的平均水平为14.99 fc。给予Miana的第2组HMGB1 mRNA表达的平均水平为13.95 fc。给予左氧氟沙星的第3组HMGB1 mRNA表达的平均水平为6.45 fc，同时给予左氧氟沙星和Miana的第4组HMGB1 mRNA表达的平均水平为5.59 fc。
Miana（(L.) Benth）在初次给药时通过调节免疫系统增加了HMGB1 mRNA表达。在[细菌名称未给出]感染后给予Miana可抑制HMGB1 mRNA表达的增加。左氧氟沙星治疗降低了HMGB1 mRNA表达水平，并且通过给予Miana叶提取物作为补充，效果得到优化。
