Li Na, Li Yangyang, Qian Chen, Liu Qing, Cao Wei, Ma Mo, He Rongbo, Chen Rourou, Geng Rong, Liu Yu
Department of Endocrinology, Sir Run Run Hospital, Nanjing Medical University, Nanjing, China.
Front Cell Infect Microbiol. 2021 Feb 16;10:624504. doi: 10.3389/fcimb.2020.624504. eCollection 2020.
Significant differences in salivary microbiota communities between polycystic ovary syndrome (PCOS) patients and healthy controls have been reported, and interestingly, some salivary microbiota exhibit diurnal oscillation in healthy people. However, whether the diurnal oscillation of salivary microbiota is present in PCOS patients is unknown. In this study, we describe the differences in the saliva microbiome between the PCOS group and the control group at different time points over 24 h. 16S rRNA gene amplicon sequencing was performed on salivary and fecal samples from 10 PCOS patients and 10 healthy controls, and salivary samples were collected at 6-h intervals over 24 h (Zeitgeber (ZT)0, ZT6, ZT12, and ZT18). Among the salivary samples, those from the PCOS group showed significant differences from those of the control group at each time point. Differences were evident in taxa level and metabolic pathways. Interestingly, we found that PCOS disrupted the diurnal rhythm of the salivary microbiota abundance, as determined in the group of healthy women. In addition, no similar changes were found in PCOS patients and controls between the oral and fecal microbiota, including differential microbiota at the phylum level. In this study, significant differences in the composition of the salivary microbiota between PCOS and healthy women were detected at different time points. We also showed that the diurnal rhythm of relative abundance of the salivary microbiota was disrupted in patients with PCOS, which might be related to development of oral-related diseases and systematic metabolic disorders.
已有报道称多囊卵巢综合征（PCOS）患者与健康对照者的唾液微生物群存在显著差异，有趣的是，一些唾液微生物群在健康人群中呈现昼夜振荡。然而，PCOS患者是否存在唾液微生物群的昼夜振荡尚不清楚。在本研究中，我们描述了PCOS组和对照组在24小时内不同时间点的唾液微生物组差异。对10例PCOS患者和10例健康对照者的唾液和粪便样本进行了16S rRNA基因扩增子测序，并在24小时内每隔6小时（授时因子（ZT）0、ZT6、ZT12和ZT18）收集唾液样本。在唾液样本中，PCOS组在每个时间点的样本与对照组均存在显著差异。在分类水平和代谢途径上差异明显。有趣的是，我们发现PCOS打乱了健康女性组中唾液微生物群丰度的昼夜节律。此外，PCOS患者和对照组的口腔和粪便微生物群之间未发现类似变化，包括门水平上的差异微生物群。在本研究中，在不同时间点检测到PCOS患者和健康女性唾液微生物群组成存在显著差异。我们还表明，PCOS患者唾液微生物群相对丰度的昼夜节律被打乱，这可能与口腔相关疾病和系统性代谢紊乱的发生有关。
