Zhang Wenying, Xin Huijie, Jiang Nannan, Lv Zhengbing, Shu Jianhong, Shi Hengbo
Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, College of Life Sciences and Medicine, Zhejiang Sci-Tech University, Hangzhou 310018, China.
College of Animal Science, Zhejiang University, Hangzhou 310058, China.
Animals (Basel). 2021 Feb 24;11(3):592. doi: 10.3390/ani11030592.
(GBacillus-9), derived from the intestinal tract of the white-spotted bamboo shark, secretes a variety of antimicrobial compounds that inhibit the growth of pathogenic bacteria. In this study, the role of GBacillus-9 in the prevention and treatment of Saanen kids with diarrhea was assessed. Six healthy kids (HL) and six kids with diarrhea (DL) were selected. All kids were fed with 0.3% (w/v) GBacillus-9 (spray power) in raw milk for two weeks. The proportion of kids with diarrhea decreased gradually as the trial progressed, and 100% DL kids were cured at day 15. GBacillus-9 increased the serum immunoglobulin (Ig) G, interleukin (IL)-4, and IL-6 concentration ( < 0.05). The amplicon sequencing analysis of the fecal bacterial community revealed that the fecal microbiota was remarkably different between the HL and the DL groups at day 0. After two weeks of feeding with GBacillus-9, no significant difference in fecal microbiota was observed between HL and DL groups at the phylum level. GBacillus-9 restored the intestinal microbial disorder associated with serum immunoglobulin and interleukin concentration. Correlation analysis showed that GBacillus-9 altered globulin and interleukin concentration and that immunoglobulin was associated with . Collectively, our results revealed that GBacillus-9 improved the gut health of kids by improving microbial homeostasis.
源自白点竹鲨肠道的GBacillus-9分泌多种抑制病原菌生长的抗菌化合物。在本研究中，评估了GBacillus-9在预防和治疗萨能山羊腹泻幼崽中的作用。选取了6只健康幼崽（HL）和6只腹泻幼崽（DL）。所有幼崽均在生牛奶中喂食0.3%（w/v）的GBacillus-9（喷雾粉剂），持续两周。随着试验进行，腹泻幼崽的比例逐渐下降，在第15天，100%的腹泻幼崽被治愈。GBacillus-9提高了血清免疫球蛋白（Ig）G、白细胞介素（IL）-4和IL-6的浓度（P<0.05）。粪便细菌群落的扩增子测序分析表明，在第0天，HL组和DL组的粪便微生物群存在显著差异。在用GBacillus-9喂食两周后，HL组和DL组在门水平上的粪便微生物群没有观察到显著差异。GBacillus-9恢复了与血清免疫球蛋白和白细胞介素浓度相关的肠道微生物紊乱。相关性分析表明，GBacillus-9改变了球蛋白和白细胞介素浓度，且免疫球蛋白与……相关。总体而言，我们的结果表明GBacillus-9通过改善微生物稳态改善了幼崽的肠道健康。
