Oh Seyeon, Shim Minjung, Son Myeongjoo, Jang Ji Tae, Son Kuk Hui, Byun Kyunghee
Functional Cellular Networks Laboratory, Department of Medicine, Graduate School and Lee Gil Ya Cancer and Diabetes Institute, College of Medicine, Gachon University, Incheon 21999, Korea.
Department of Anatomy & Cell Biology, Gachon University College of Medicine, Incheon 21936, Korea.
Antioxidants (Basel). 2021 Feb 16;10(2):298. doi: 10.3390/antiox10020298.
Disruptions of the Treg/Th17 cell balance and gut barrier function are associated with endothelial dysfunction. Dieckol (DK) obtained from and extract (ECE) decreases blood pressure by reducing inflammation; however, it has not been elucidated whether DK or ECE modulates the Treg/Th17 balance, changes the gut epithelial barrier, or decreases endothelial cell dysfunction. We evaluated the effects of ECE and DK on gut barrier and the Treg/Th17 balance in the intestine and aorta, with regard to endothelial dysfunction, using the spontaneously hypertensive rat (SHR) model. The level of Th17 cells increased and that of Treg cells decreased in the intestine of SHRs compared to normotensive Wistar Kyoto (WKY) rat. These changes were attenuated by ECE or DK treatment. Additionally, the serum IL-17A level increased in SHRs more than WKY; this was decreased by ECE or DK treatment. The level of Treg cells decreased and that of Th17 cells increased in the aorta of SHRs. These changes were attenuated by ECE or DK treatment. The NF-κB and IL-6 levels were increased in SHRs, but these changes were reversed by ECE or DK treatment. Endothelial cell dysfunction, which was evaluated using peNOS/eNOS, nitrate/nitrite ratio, and NADPH oxidase activity, increased in the aorta of SHRs, but was decreased by ECE or DK treatment. The Treg/Th17 balance in the intestine and aorta of SHRs was attenuated and endothelial cell dysfunction was attenuated through the Th17/NF-κB/IL-6 pathway by ECE or DK.
调节性T细胞（Treg）/辅助性T细胞17（Th17）细胞平衡的破坏和肠道屏障功能与内皮功能障碍有关。从裙带菜和海带提取物（ECE）中获得的二eck醇（DK）通过减轻炎症来降低血压；然而，DK或ECE是否调节Treg/Th17平衡、改变肠道上皮屏障或减少内皮细胞功能障碍尚未阐明。我们使用自发性高血压大鼠（SHR）模型评估了ECE和DK对肠道屏障以及肠道和主动脉中Treg/Th17平衡的影响，以及对内皮功能障碍的影响。与血压正常的Wistar Kyoto（WKY）大鼠相比，SHR肠道中Th17细胞水平升高，Treg细胞水平降低。ECE或DK治疗可减轻这些变化。此外，SHR血清白细胞介素-17A（IL-17A）水平比WKY大鼠升高得更多；ECE或DK治疗可使其降低。SHR主动脉中Treg细胞水平降低，Th17细胞水平升高。ECE或DK治疗可减轻这些变化。SHR中核因子κB（NF-κB）和IL-6水平升高，但ECE或DK治疗可使其逆转。使用磷酸化内皮型一氧化氮合酶（peNOS）/内皮型一氧化氮合酶（eNOS）、硝酸盐/亚硝酸盐比值和烟酰胺腺嘌呤二核苷酸磷酸（NADPH）氧化酶活性评估的内皮细胞功能障碍在SHR主动脉中增加，但ECE或DK治疗可使其降低。ECE或DK通过Th17/NF-κB/IL-6途径减轻了SHR肠道和主动脉中的Treg/Th17平衡，并减轻了内皮细胞功能障碍。
