Department of Rheumatology and Inflammation Research, Institute of Medicine, The Sahlgrenska Academy at University of Gothenburg, 413 46 Gothenburg, Sweden.
Wallenberg Centre for Molecular and Translational Medicine at the University of Gothenburg, 405 30 Gothenburg, Sweden.
Biomolecules. 2021 Feb 21;11(2):325. doi: 10.3390/biom11020325.
Adiponectin, leptin, and resistin are adipocytokines whose levels are elevated in blood and synovial fluid from patients with rheumatoid arthritis (RA). However, their role in RA pathogenesis is unclear. Here, we examined whether adipocytokines are associated with circulating chemokines, markers of inflammation and RA disease activity in patients with untreated newly diagnosed RA. Plasma levels of 15 chemokines, adiponectin, leptin, and resistin were measured using flow cytometry bead-based immunoassay or enzyme-linked immunosorbent assay (ELISA) in a cohort of 70 patients with untreated newly diagnosed RA. Markers of inflammation and disease activity were also assessed in all patients. Positive association was found between total adiponectin and CXCL10 (β = 0.344, = 0.021), CCL2 (β = 0.342, = 0.012), and CXCL9 (β = 0.308, = 0.044), whereas high-molecular weight (HMW) adiponectin associated only with CXCL9 (β = 0.308, = 0.033). Furthermore, both total and HMW adiponectin were associated with C-reactive protein (β = 0.485, = 0.001; β = 0.463, = 0.001) and erythrocyte sedimentation rate (β = 0.442, = 0.001; β = 0.507, < 0.001). Leptin and resistin were not associated with plasma chemokines, markers of inflammation, or disease activity scores. Our study shows an association between circulating adiponectin and pro-inflammatory chemokines involved in RA pathogenesis as well as markers of inflammation in a well-characterized cohort of patients with untreated newly diagnosed RA.
脂联素、瘦素和抵抗素是脂肪细胞因子，其在类风湿关节炎 (RA) 患者的血液和滑液中水平升高。然而，它们在 RA 发病机制中的作用尚不清楚。在这里，我们研究了脂肪细胞因子是否与未经治疗的初诊 RA 患者的循环趋化因子、炎症标志物和 RA 疾病活动相关。使用流式细胞术基于珠的免疫测定法或酶联免疫吸附测定法 (ELISA)，在 70 名未经治疗的初诊 RA 患者的队列中测量了 15 种趋化因子、脂联素、瘦素和抵抗素的血浆水平。在所有患者中还评估了炎症和疾病活动标志物。发现总脂联素与 CXCL10（β=0.344，P=0.021）、CCL2（β=0.342，P=0.012）和 CXCL9（β=0.308，P=0.044）呈正相关，而高分子量 (HMW) 脂联素仅与 CXCL9 相关（β=0.308，P=0.033）。此外，总脂联素和 HMW 脂联素与 C 反应蛋白（β=0.485，P=0.001；β=0.463，P=0.001）和红细胞沉降率（β=0.442，P=0.001；β=0.507，P<0.001）呈正相关。瘦素和抵抗素与血浆趋化因子、炎症标志物或疾病活动评分无关。我们的研究表明，在未经治疗的初诊 RA 患者的特征明确的队列中，循环脂联素与参与 RA 发病机制的促炎趋化因子以及炎症标志物之间存在关联。
