类风湿关节炎患者滑膜和滑液中Nesfatin-1水平升高与促炎细胞因子有关。
Elevated Nesfatin-1 Level in Synovium and Synovial Fluid is Associated with Pro-Inflammatory Cytokines in Patients with Rheumatoid Arthritis.
作者信息
Zhang Shuo, Rong Genxiang, Xu Yayun, Jing Juehua
机构信息
Department of Orthopedics, The Second Affiliated Hospital of Anhui Medical University, Hefei, People's Republic of China.
Department of Orthopedics, The First Affiliated Hospital of Anhui Medical University, Hefei, People's Republic of China.
出版信息
Int J Gen Med. 2021 Sep 7;14:5269-5278. doi: 10.2147/IJGM.S330099. eCollection 2021.
BACKGROUND
Adipocytokines have been proven to be involved in the progression of autoimmune diseases, including rheumatoid arthritis (RA). Nesfatin-1, a newly discovered adipokine, has recently been reported to possess potent anti-inflammatory, antiapoptotic, and antioxidative properties. However, its role in RA has not yet been reported. Therefore, this study aimed to determine nesfatin-1 levels in the synovium and synovial fluid (SF) of patients with RA and examine their correlation with clinical manifestations and proinflammatory cytokine levels.
METHODS
Synovium and SF samples were collected from patients with RA and non-RA patients during joint surgery. Immunohistochemistry was used to measure nesfatin-1 protein expression in the synovium. Enzyme-linked immunosorbent assay was used to measure nesfatin-1, interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) levels in the synovium and SF. Pearson correlation analysis was used to evaluate the correlations between nesfatin-1 levels, RA clinical features, and proinflammatory cytokines. The diagnostic value of synovium nesfatin-1 for RA was assessed using receiver operating characteristic (ROC) curve analysis.
RESULTS
The results showed that nesfatin-1, IL-1β, and TNF-α levels in the synovium were significantly higher in patients with RA than in controls, with age and body mass index as covariates. Moreover, the results of Pearson correlation analysis showed that nesfatin-1 levels were positively correlated with IL-1β and TNF-α levels in the synovium of patients with RA. Furthermore, there was a positive relationship between synovium nesfatin-1 levels and rheumatoid factor in patients with RA. Additionally, the results of the ROC curve analysis revealed an area under the curve of 0.733 with 77.5% sensitivity and 60.0% specificity for synovium nesfatin-1 in discriminating patients with RA from controls.
CONCLUSION
These findings suggest that increased nesfatin-1 levels in the synovium may be associated with proinflammatory cytokines and RA severity.
背景
脂肪细胞因子已被证明参与包括类风湿关节炎（RA）在内的自身免疫性疾病的进展。Nesfatin-1是一种新发现的脂肪细胞因子，最近有报道称其具有强大的抗炎、抗凋亡和抗氧化特性。然而，其在RA中的作用尚未见报道。因此，本研究旨在测定RA患者滑膜和滑液（SF）中nesfatin-1的水平，并探讨其与临床表现及促炎细胞因子水平的相关性。
方法
在关节手术期间从RA患者和非RA患者中采集滑膜和SF样本。采用免疫组织化学法检测滑膜中nesfatin-1蛋白表达。采用酶联免疫吸附测定法检测滑膜和SF中nesfatin-1、白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的水平。采用Pearson相关性分析评估nesfatin-1水平、RA临床特征和促炎细胞因子之间的相关性。使用受试者工作特征（ROC）曲线分析评估滑膜nesfatin-1对RA的诊断价值。
结果
结果显示，以年龄和体重指数作为协变量，RA患者滑膜中nesfatin-1、IL-1β和TNF-α水平显著高于对照组。此外，Pearson相关性分析结果显示，RA患者滑膜中nesfatin-1水平与IL-1β和TNF-α水平呈正相关。此外，RA患者滑膜nesfatin-1水平与类风湿因子之间存在正相关关系。此外，ROC曲线分析结果显示，滑膜nesfatin-1区分RA患者与对照组的曲线下面积为0.733，敏感性为77.5%，特异性为60.0%。
结论
这些发现表明，滑膜中nesfatin-1水平升高可能与促炎细胞因子及RA严重程度相关。
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