Attia Mohamed S, Osman Mahmoud S, Mohamed Amr S, Mahgoub Hany A, Garada Mohamed O, Abdelmouty Eslam S, Abdel Latef Arafat Abdel Hamed
Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11651, Egypt.
Biotechnology Lab, Horticulture Research Institute, Agricultural Research Center, Giza 12619, Egypt.
Plants (Basel). 2021 Feb 18;10(2):388. doi: 10.3390/plants10020388.
In this study, the anti-stress capabilities of the foliar application of chitosan, dissolved in four different organic acids (acetic acid, ascorbic acid, citric acid and malic acid) have been investigated on tomato ( L.) plants under salinity stress (100 mM NaCl). Morphological traits, photosynthetic pigments, osmolytes, secondary metabolites, oxidative stress, minerals, antioxidant enzymes activity, isozymes and protein patterns were tested for potential tolerance of tomato plants growing under salinity stress. Salinity stress was caused a reduction in growth parameters, photosynthetic pigments, soluble sugars, soluble proteins and potassium (K) content. However, the contents of proline, ascorbic acid, total phenol, malondialdehyde (MDA), hydrogen peroxide (HO), sodium (Na) and antioxidant enzyme activity were increased in tomato plants grown under saline conditions. Chitosan treatments in any of the non-stressed plants showed improvements in morphological traits, photosynthetic pigments, osmolytes, total phenol and antioxidant enzymes activity. Besides, the harmful impacts of salinity on tomato plants have also been reduced by lowering MDA, HO and Na levels. Chitosan treatments in either non-stressed or stressed plants showed different responses in number and density of peroxidase (POD), polyphenol oxidase (PPO) and superoxide dismutase (SOD) isozymes. NaCl stress led to the diminishing of protein bands with different molecular weights, while they were produced again in response to chitosan foliar application. These responses were varied according to the type of solvent acid. It could be suggested that foliar application of chitosan, especially that dissolved in ascorbic or citric acid, could be commercially used for the stimulation of tomato plants grown under salinity stress.
在本研究中，已对在盐胁迫（100 mM NaCl）下的番茄（L.）植株，研究了叶面喷施溶解于四种不同有机酸（乙酸、抗坏血酸、柠檬酸和苹果酸）中的壳聚糖的抗胁迫能力。对盐胁迫下生长的番茄植株的形态特征、光合色素、渗透调节物质、次生代谢产物、氧化应激、矿物质、抗氧化酶活性、同工酶和蛋白质模式进行了潜在耐受性测试。盐胁迫导致生长参数、光合色素、可溶性糖、可溶性蛋白质和钾（K）含量降低。然而，在盐胁迫条件下生长的番茄植株中，脯氨酸、抗坏血酸、总酚、丙二醛（MDA）、过氧化氢（HO）、钠（Na）含量和抗氧化酶活性增加。在任何非胁迫植株中进行壳聚糖处理均显示出形态特征、光合色素、渗透调节物质、总酚和抗氧化酶活性有所改善。此外，通过降低MDA、HO和Na水平，盐胁迫对番茄植株的有害影响也有所减轻。在非胁迫或胁迫植株中进行壳聚糖处理，过氧化物酶（POD）、多酚氧化酶（PPO）和超氧化物歧化酶（SOD）同工酶的数量和密度表现出不同的响应。NaCl胁迫导致不同分子量的蛋白条带减少，而叶面喷施壳聚糖后这些条带又重新出现。这些响应因溶剂酸的类型而异。可以认为，叶面喷施壳聚糖，尤其是溶解于抗坏血酸或柠檬酸中的壳聚糖，可商业化用于刺激在盐胁迫下生长的番茄植株。
