College of Landscape Architecture, Sichuan Agricultural University, Wenjiang 611130, China.
Department of Plant Sciences, University of California Davis, Davis, CA 95616, USA.
Int J Mol Sci. 2022 Jul 24;23(15):8155. doi: 10.3390/ijms23158155.
is the only woody resurrection plant found in the world and can survive from long-term desiccation. Therefore, could be considered as a valuable resource for study of plant adaptation to abiotic stress. However, few genes related to its drought tolerance have been functionally characterized and the molecular mechanisms underlying the stress tolerance of are largely unknown. The phytochrome interacting factor (PIF) family is a group of basic helix-loop-helix (bHLH) transcription factors and functions as the core regulator in plant growth and development. However, less is known of its participation in abiotic stress response. In this study, we isolated and characterized a dehydration-inducible PIF gene from . Heterologous expression of in Arabidopsis enhanced tolerance to drought and salinity stresses at seedling and adult stages. It significantly increased primary root length and stomatal aperture (ration of length/width) under stress treatments and decreased water loss rate. Compared with WT, the transgenic lines overexpressing exhibited higher chlorophyll content and lower malondialdehyde accumulation. The abilities of osmotic adjustment and reactive oxygen species scavenging were also enhanced in transgenic lines. These results suggest that MfPIF8 may participate in the positive regulation of abiotic stress responses. Additional investigation of its mechanism is needed in the future.
是世界上唯一发现的木本复苏植物，能够从长期干旱中存活下来。因此， 可以被认为是研究植物适应非生物胁迫的有价值的资源。然而，与耐旱性相关的基因很少被功能表征，其对胁迫的分子机制在很大程度上尚不清楚。光敏色素相互作用因子 (PIF) 家族是一类碱性螺旋-环-螺旋 (bHLH) 转录因子，作为植物生长发育的核心调节剂发挥作用。然而，人们对其参与非生物胁迫反应的了解较少。在这项研究中，我们从 中分离并鉴定了一个脱水诱导的 PIF 基因 。 在拟南芥中的异源表达增强了幼苗和成年期对干旱和盐胁迫的耐受性。它在胁迫处理下显著增加了主根长度和气孔开度（长度/宽度比），并降低了失水率。与 WT 相比，过表达 的转基因系表现出更高的叶绿素含量和更低的丙二醛积累。渗透调节和活性氧清除的能力也在 转基因系中得到增强。这些结果表明，MfPIF8 可能参与了非生物胁迫反应的正向调节。未来还需要进一步研究其机制。
