Cuenca Maigualida, Sánchez María Carmen, Diz Pedro, Martínez-Lamas Lucía, Álvarez Maximiliano, Limeres Jacobo, Sanz Mariano, Herrera David
ETEP (Etiology and Therapy of Periodontal and Peri-Implant Diseases) Research Group, University Complutense of Madrid (UCM), 28040 Madrid, Spain.
Medical-Surgical Dentistry Research Group (OMEQUI), Health Research Institute of Santiago de Compostela (IDIS), University of Santiago de Compostela (USC), 15705 Santiago de Compostela, Spain.
Microorganisms. 2021 Feb 22;9(2):450. doi: 10.3390/microorganisms9020450.
The aim of this study was to evaluate the potential anti-biofilm and antibacterial activities of sp. nov. To test anti-biofilm properties, and were grown in a biofilm model in the presence or not of sp. nov. for up to 120 h. For the potential antibacterial activity, 24 h-biofilms were exposed to sp. nov for 24 and 48 h. Biofilms structures and bacterial viability were studied by microscopy, and the effect in bacterial load by quantitative polymerase chain reaction. A generalized linear model was constructed, and results were considered as statistically significant at < 0.05. The presence of sp. nov. during biofilm development did not affect the structure of the community, but an anti-biofilm effect against was observed ( < 0.001, after 96 and 120 h). For antibacterial activity, after 24 h of exposure to sp. nov., counts of ( = 0.019) and ( = 0.020) were significantly reduced in well-structured biofilms. Although moderate, anti-biofilm and antibacterial activities of sp. nov. against oral bacteria, including some periodontal pathogens, were demonstrated in an in vitro biofilm model.
本研究的目的是评估新种的潜在抗生物膜和抗菌活性。为测试抗生物膜特性，在有或无新种的情况下，将其在生物膜模型中培养长达120小时。对于潜在的抗菌活性，将24小时的生物膜暴露于新种中24小时和48小时。通过显微镜研究生物膜结构和细菌活力，并通过定量聚合酶链反应研究对细菌载量的影响。构建了广义线性模型，结果在P<0.05时被认为具有统计学意义。在生物膜形成过程中新种的存在并未影响群落结构，但观察到对其有抗生物膜作用（96小时和120小时后，P<0.001）。对于抗菌活性，在暴露于新种24小时后，结构良好的生物膜中（P = 0.019）和（P = 0.020）的计数显著减少。尽管作用中等，但新种对口腔细菌（包括一些牙周病原体）的抗生物膜和抗菌活性在体外生物膜模型中得到了证实。
