Buonfrate Dora, Marrone Rosalia, Silva Ronaldo, Mirisola Concetta, Ragusa Andrea, Mistretta Manuela, Perandin Francesca, Bisoffi Zeno
Department of Infectious Tropical Diseases and Microbiology (DITM), IRCCS Sacro Cuore Don Calabria Hospital, 37024 Negrar, Italy.
National Institute for Health Migration and Poverty (INMP), 00153 Rome, Italy.
Microorganisms. 2021 Feb 15;9(2):401. doi: 10.3390/microorganisms9020401.
infection is a life-threatening neglected tropical disease. Diagnostic issues have caused an underestimation of its global burden. The choice of appropriate diagnostic tests for the screening of populations at risk of the infection, such as migrants from endemic countries, is of paramount importance. From November 2017 to July 2018, all migrants presenting to the National Institute for Health Migration and Poverty (INMP) in Rome, Italy were offered screening tests for infection. The study objective was to estimate the prevalence of strongyloidiasis in the study population and the accuracy of a novel ELISA assay. The following tests were carried out at the IRCCS Sacro Cuore Don Calabria hospital in Negrar, Verona: stool microscopy, real-time PCR for , in-house immunofluorescence test (IFAT), a commercial ELISA assay (Bordier ELISA), and a novel ELISA assay (Euroimmun ELISA). A latent class analysis (LCA) model set up with test results, clinical variables, and eosinophilia indicated a prevalence around 7.5%, in line with previous findings. The sensitivity and the specificity of Euroimmun ELISA were 90.6% (95% CI 80.5-100) and 87.7% (95CI 84.5-91.0); these results indicate that the novel ELISA assay would be suitable for screening of migrants from endemic countries.
感染是一种危及生命的被忽视热带病。诊断问题导致对其全球负担的低估。为有感染风险的人群（如来自流行国家的移民）选择合适的诊断测试至关重要。2017年11月至2018年7月，所有前往意大利罗马国家卫生移民与贫困研究所（INMP）的移民都接受了感染筛查测试。该研究的目的是估计研究人群中粪类圆线虫病的患病率以及一种新型酶联免疫吸附测定（ELISA）的准确性。在维罗纳内格拉尔的IRCCS Sacro Cuore Don Calabria医院进行了以下测试：粪便显微镜检查、针对[具体病原体未明确]的实时聚合酶链反应（PCR）、内部免疫荧光试验（IFAT）、一种商业ELISA测定（Bordier ELISA）以及一种新型ELISA测定（Euroimmun ELISA）。利用测试结果、临床变量和嗜酸性粒细胞增多症建立的潜在类别分析（LCA）模型表明患病率约为7.5%，与先前的研究结果一致。Euroimmun ELISA的敏感性和特异性分别为90.6%（95%可信区间80.5 - 100）和87.7%（95%可信区间84.5 - 91.0）；这些结果表明这种新型ELISA测定适用于对来自流行国家的移民进行筛查。
