Souza Adriana Oliveira, Oliveira Johny Wysllas de Freitas, Moreno Claudia Jéssica Gonsalves, de Medeiros Mayra Jane Campos, Fernandes-Negreiros Marília Medeiros, Souza Flavia Roberta Monteiro, Pontes Daniel Lima, Silva Marcelo Sousa, Rocha Hugo Alexandre Oliveira
Laboratório de Biotecnologia de Polímeros Naturais (BIOPOL), Departamento de Bioquímica, Centro de Biociências, Universidade Federal do Rio Grande do Norte (UFRN), Natal 59078-970, RN, Brazil.
Laboratório de Imunoparasitologia, Departamento de Análises Clínicas e Toxicológicas, Centro de Ciências da Saúde, Universidade Federal do Rio Grande do Norte, Natal 59012-570, RN, Brazil.
Nanomaterials (Basel). 2022 Jun 15;12(12):2059. doi: 10.3390/nano12122059.
The brown seaweed synthesizes three bioactive fucoidans, the most abundant of which is fucan A. This fucoidan was extracted and its identity was confirmed by chemical analysis, Fourier-transform infrared spectroscopy (FTIR), and agarose gel electrophoresis. Thereafter, silver nanoparticles containing fucan A (AgFuc) were produced using an environmentally friendly synthesis method. AgFuc synthesis was analyzed via UV-vis spectroscopy and FTIR, which confirmed the presence of both silver and fucan A in the AgFuc product. Dynamic light scattering (DLS), X-ray diffraction, scanning electron microscopy, and atomic force microscopy revealed that the AgFuc particles were ~180.0 nm in size and spherical in shape. DLS further demonstrated that AgFuc was stable for five months. Coupled plasma optical emission spectrometry showed that the AgFuc particles contained 5% silver and 95% sugar. AgFuc was shown to be more effective in inhibiting the ability of parasites to reduce MTT than fucan A or silver, regardless of treatment time. In addition, AgFuc induced the death of ~60% of parasites by necrosis and ~17% by apoptosis. Therefore, AgFuc induces damage to the parasites' mitochondria, which suggests that it is an anti- agent. This is the first study to analyze silver nanoparticles containing fucan as an anti-Trypanosoma cruzi agent. Our data indicate that AgFuc nanoparticles have potential therapeutic applications, which should be determined via preclinical in vitro and in vivo studies.
棕色海藻合成了三种生物活性岩藻聚糖，其中含量最高的是岩藻聚糖A。提取了这种岩藻聚糖，并通过化学分析、傅里叶变换红外光谱（FTIR）和琼脂糖凝胶电泳确认了其身份。此后，使用一种环境友好的合成方法制备了含有岩藻聚糖A的银纳米颗粒（AgFuc）。通过紫外可见光谱和FTIR对AgFuc的合成进行了分析，证实了AgFuc产物中同时存在银和岩藻聚糖A。动态光散射（DLS）、X射线衍射、扫描电子显微镜和原子力显微镜显示，AgFuc颗粒的尺寸约为180.0 nm，呈球形。DLS进一步表明，AgFuc在五个月内保持稳定。电感耦合等离子体发射光谱显示，AgFuc颗粒含有5%的银和95%的糖。无论处理时间如何，AgFuc在抑制寄生虫还原MTT的能力方面都比岩藻聚糖A或银更有效。此外，AgFuc通过坏死诱导约60%的寄生虫死亡，通过凋亡诱导约17%的寄生虫死亡。因此，AgFuc会对寄生虫的线粒体造成损伤，这表明它是一种抗寄生虫剂。这是第一项分析含有岩藻聚糖的银纳米颗粒作为抗克氏锥虫剂的研究。我们的数据表明，AgFuc纳米颗粒具有潜在的治疗应用价值，这应该通过临床前的体外和体内研究来确定。
