Wang Yineng, Cao Xi, Messina Walter, Hogan Anna, Ugwah Justina, Alatawi Hanan, van Zalen Ed, Moore Eric
Life Science Interface Group, Tyndall National Institute, University College Cork, T12 R5CP Cork, Ireland.
Chemistry Department, University College Cork, T12 K8AF Cork, Ireland.
Micromachines (Basel). 2021 Feb 27;12(3):239. doi: 10.3390/mi12030239.
Capillary electrochromatography (CEC) is a separation technique that hybridizes liquid chromatography (LC) and capillary electrophoresis (CE). The selectivity offered by LC stationary phase results in rapid separations, high efficiency, high selectivity, minimal analyte and buffer consumption. Chip-based CE and CEC separation techniques are also gaining interest, as the microchip can provide precise on-chip control over the experiment. Capacitively coupled contactless conductivity detection (CD) offers the contactless electrode configuration, and thus is not in contact with the solutions under investigation. This prevents contamination, so it can be easy to use as well as maintain. This study investigated a chip-based CE/CEC with CD technique, including silicon-based microfluidic device fabrication processes with packaging, design and optimization. It also examined the compatibility of the silicon-based CEC microchip interfaced with CD. In this paper, the authors demonstrated a nanofabrication technique for a novel microchip electrochromatography (MEC) device, whose capability is to be used as a mobile analytical equipment. This research investigated using samples of potassium ions, sodium ions and aspirin (acetylsalicylic acid).
毛细管电色谱（CEC）是一种将液相色谱（LC）和毛细管电泳（CE）相结合的分离技术。液相色谱固定相所提供的选择性使得分离快速、高效、高选择性，且分析物和缓冲液消耗极少。基于芯片的CE和CEC分离技术也越来越受到关注，因为微芯片能够对实验进行精确的芯片上控制。电容耦合非接触式电导检测（CD）提供了非接触式电极配置，因此不与被研究的溶液接触。这可防止污染，所以易于使用和维护。本研究考察了一种基于芯片的采用CD技术的CE/CEC，包括具有封装、设计和优化的硅基微流控器件制造工艺。它还研究了硅基CEC微芯片与CD联用的兼容性。在本文中，作者展示了一种用于新型微芯片电色谱（MEC）器件的纳米制造技术，该器件能够用作移动分析设备。本研究使用钾离子、钠离子和阿司匹林（乙酰水杨酸）样本进行了研究。
