State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, 800 Dongchuan Road, Shanghai 200240, China.
Joint International Research Laboratory of Metabolic & Developmental Sciences, Shanghai Jiao Tong University, Shanghai 200240, China.
Int J Mol Sci. 2021 Feb 27;22(5):2387. doi: 10.3390/ijms22052387.
Saline-alkali soil has become an important environmental problem for crop productivity. One of the most effective approaches is to cultivate new stress-tolerant plants through genetic engineering. Through RNA-seq analysis and RT-PCR validation, a novel bZIP transcription factor ChbZIP1, which is significantly upregulated at alkali conditions, was obtained from alkaliphilic microalgae sp. BLD. Overexpression of ChbZIP1 in and increased their alkali resistance, indicating ChbZIP1 may play important roles in alkali stress response. Through subcellular localization and transcriptional activation activity analyses, we found that ChbZIP1 is a nuclear-localized bZIP TF with transactivation activity to bind with the motif of G-box 2 (TGACGT). Functional analysis found that genes such as GPX1, DOX1, CAT2, and EMB, which contained G-box 2 and were associated with oxidative stress, were significantly upregulated in with ChbZIP1 overexpression. The antioxidant ability was also enhanced in transgenic . These results indicate that ChbZIP1 might mediate plant adaptation to alkali stress through the active oxygen detoxification pathway. Thus, ChbZIP1 may contribute to genetically improving plants' tolerance to alkali stress.
盐碱土已成为影响作物生产力的重要环境问题。其中一种最有效的方法是通过基因工程培育新的耐盐植物。通过 RNA-seq 分析和 RT-PCR 验证，从嗜碱微藻 sp. BLD 中获得了一种新型 bZIP 转录因子 ChbZIP1，该转录因子在碱胁迫条件下显著上调。在 和 中过表达 ChbZIP1 提高了它们的耐碱性，表明 ChbZIP1 可能在碱胁迫响应中发挥重要作用。通过亚细胞定位和转录激活活性分析，发现 ChbZIP1 是一种具有核定位的 bZIP TF，具有与 G-box 2 (TGACGT) 结合的转录激活活性。功能分析发现，在过表达 ChbZIP1 的 中，含有 G-box 2 并与氧化应激相关的基因如 GPX1、DOX1、CAT2 和 EMB 显著上调。转基因 的抗氧化能力也得到增强。这些结果表明，ChbZIP1 可能通过活性氧解毒途径介导植物对碱胁迫的适应。因此，ChbZIP1 可能有助于通过遗传改良提高植物对碱胁迫的耐受性。
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