Guselnikova Olga, Lim Hyunsoo, Na Jongbeom, Eguchi Miharu, Kim Hyun-Jong, Elashnikov Roman, Postnikov Pavel, Svorcik Vaclav, Semyonov Oleg, Miliutina Elena, Lyutakov Oleksiy, Yamauchi Yusuke
Department of Solid State Engineering, University of Chemistry and Technology, 16628, Prague, Czech Republic; Research School of Chemistry and Applied Biomedical Sciences, Tomsk Polytechnic University, 634049, Tomsk, Russian Federation.
Australian Institute for Bioengineering and Nanotechnology (AIBN), The University of Queensland, Brisbane, QLD, 4072, Australia; New & Renewable Energy Research Center, Korea Electronics Technology Institute (KETI), 25, Saenari-ro, Bundang-gu, Seongnam-si, Gyeonggi-do, 13509, Republic of Korea.
Biosens Bioelectron. 2021 May 15;180:113109. doi: 10.1016/j.bios.2021.113109. Epub 2021 Feb 24.
Here, we present a new family of hierarchical porous hybrid materials as an innovative tool for ultrasensitive and selective sensing of enantiomeric drugs in complex biosamples via chiral surface-enhanced Raman spectroscopy (SERS). Hierarchical porous hybrid films were prepared by the combination of mesoporous plasmonic Au films and microporous homochiral metal-organic frameworks (HMOFs). The proposed hierarchical porous substrates enable extremely low limit of detection values (10 M) for pseudoephedrine in undiluted blood plasma due to dual enhancement mechanisms (physical enhancement by the mesoporous Au nanostructures and chemical enhancement by HMOF), chemical recognition by HMOF, and a discriminant function for bio-samples containing large biomolecules, such as blood components. We demonstrate the effect of each component (mesoporous Au and microporous AlaZnCl (HMOF)) on the analytical performance for sensing. The growth of AlaZnCl leads to an increase in the SERS signal (by around 17 times), while the use of mesoporous Au leads to an increase in the signal (by up to 40%). In the presence of a complex biomatrix (blood serum or plasma), the hybrid hierarchical porous substrate provides control over the transport of the molecules inside the pores and prevents blood protein infiltration, provoking competition with existing plasmonic materials at the limit of detection and enantioselectivity in the presence of a multicomponent biomatrix.
在此，我们展示了一类新型的分级多孔杂化材料，作为一种创新工具，可通过手性表面增强拉曼光谱（SERS）对复杂生物样品中的对映体药物进行超灵敏和选择性传感。分级多孔杂化膜是通过介孔等离子体金膜与微孔手性金属有机框架（HMOF）相结合制备而成。所提出的分级多孔基底由于双重增强机制（介孔金纳米结构的物理增强和HMOF的化学增强）、HMOF的化学识别以及对含有大生物分子（如血液成分）的生物样品的判别功能，使得在未稀释的血浆中伪麻黄碱的检测限极低（10⁻¹¹ M）。我们展示了各组分（介孔金和微孔AlaZnCl（HMOF））对传感分析性能的影响。AlaZnCl的生长导致SERS信号增强（约17倍），而使用介孔金则使信号增强（高达40%）。在存在复杂生物基质（血清或血浆）的情况下，分级多孔杂化基底可控制分子在孔内的传输，并防止血液蛋白渗入，从而避免在检测限和对映选择性方面与现有等离子体材料在多组分生物基质存在时产生竞争。
