Li Lin, Liu Yuanchu, Huang Ying, Li Bin, Ma Wen, Wang Donghao, Cao Xiaoyan, Wang Zhezhi
National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest of China, Key Laboratory of the Ministry of Education for Medicinal Resources and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi'an, China.
Front Plant Sci. 2021 Feb 18;12:630424. doi: 10.3389/fpls.2021.630424. eCollection 2021.
Bunge (), a traditional Chinese medicinal herb, contains numerous bioactive components with broad range of pharmacological properties. By increasing the levels of endogenous jasmonate (JA) in plants or treating them with methyl jasmonate (MeJA), the level of tanshinones and salvianolic acids can be greatly enhanced. The jasmonate ZIM (JAZ) proteins belong to the TIFY family, and act as repressors, releasing targeted transcriptional factors in the JA signaling pathway. Herein, we identified and characterized 15 TIFY proteins present in . Quantitative reverse transcription PCR analysis indicated that the genes were all constitutively expressed in different tissues and were induced by MeJA treatments. SmJAZ3, which negatively regulates the tanshinones biosynthesis pathway in and the detailed molecular mechanism is poorly understood. SmJAZ3 acts as a bait protein to capture and identify a WD-repeat containing the protein SmWD40-170. Further molecular and genetic analysis revealed that SmWD40-170 is a positive regulator, promoting the accumulation of secondary metabolites in . Our study systematically analyzed the TIFY family and speculated a module of the JAZ-WD40 complex provides new insights into the mechanisms regulating the biosynthesis of secondary metabolites in .
丹参是一种传统中药材，含有多种具有广泛药理特性的生物活性成分。通过提高植物体内内源茉莉酸（JA）水平或用茉莉酸甲酯（MeJA）处理植物，丹参酮和丹酚酸的含量可大幅提高。茉莉酸ZIM（JAZ）蛋白属于TIFY家族，作为阻遏物，在JA信号通路中释放靶向转录因子。在此，我们鉴定并表征了丹参中存在的15种TIFY蛋白。定量逆转录PCR分析表明，这些基因在不同组织中均组成型表达，并受MeJA处理诱导。SmJAZ3负调控丹参中丹参酮生物合成途径，其详细分子机制尚不清楚。SmJAZ3作为诱饵蛋白捕获并鉴定了一种含WD重复序列的蛋白SmWD40 - 170。进一步的分子和遗传分析表明，SmWD40 - 170是一个正调控因子，促进丹参中次生代谢产物的积累。我们的研究系统分析了TIFY家族，并推测了JAZ - WD40复合物模块，为丹参中次生代谢产物生物合成调控机制提供了新见解。
